微小RNA在结肠慢传输型便秘病变组织中的差异表达  

Differential expression of microRNAs in colonic slow transit constipation

作　　者：汪名飞[1] 刘志苏[1] 江从庆[1] 宋惊喜[1] 丁召[1] 唐胜利[1] 钱群[1] 

机构地区：[1]武汉大学中南医院结直肠肛门外科湖北省肠病医学l临床研究中心肠病湖北省重点实验室,武汉430071

出　　处：《中华实验外科杂志》2012年第2期217-218,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察微小RNA（miRNA）在结肠慢传输型便秘（STC）病变结肠组织和正常结肠组织中的差异表达。方法收集6例STC病变结肠组织和正常结肠组织标本，应用miRNA芯片检测miRNA在STC病变结肠组织和正常结肠组织中的表达。结果5种miRNA在STC病变结肠组织正常结肠组织中的表达差异有统计学意义（P〈0．05）。与正常结肠组织比较，miR-20b、miR-27b、miR-30b、miR．128及miR-129．3p在STC结肠组织中表达下调，统计学组间差异倍数均在1．2倍以上，其中miR．128及miR．129．3p下调明显，统计学组间差异倍数大于2倍。结论部分miRNA在STC结肠组织和正常结肠组织中存在差异表达，可能参与了STC发生的分子机制。Objective To explore the micmRNA （miRNA） differential expression in the patients with colonic slow transit constipation （STC）, and to provide the evidence that miRNA may be involved in the molecular pathogensis of STC. Methods Six colonic STC tissue samples and 6 normal coloic tissue samples were obtained surgically, miRNA expression was assessed using miRNA microarray. Results miRNA microarray identified five miRNA, （miR-20b, miR-27b, miR-30b, miR-128 and miR-129-3p）, with down-regulated expression （ over 1.2-fold lower） in colonic STC tissues compared to normal colonic tissues （P 〈 0. 05）. miR-128 and miR-129-3p were more than 2-fold decreased respectively. Conclusion Some miRNA are expressed differently between colonic STC tissues and normal colonic tissues, which may be involved in the pathogensis of STC.

关 键 词：微小RNA 慢传输型便秘 芯片 

分 类 号：R574.62[医药卫生—消化系统]