激活的T细胞核因子家族在肝癌与癌旁组织中的差异表达  

Differential exoression of nuclear factor of activated T cell gene family member mRNA in hepatoceilular carcinoma tissues and adjacent non-tumorous tissues

作　　者：张进[1] 刘志苏[1] 刘权焰[1] 龚铖[1] 刘阳[1] 孙江阳[1] 

机构地区：[1]武汉大学中南医院普外科,武汉430071

出　　处：《中华实验外科杂志》2012年第2期237-239,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30872491/C160402、81172349）

摘　　要：目的观察30例患者肝癌与邻近癌旁组织中激活的T细胞核因子（NFAT）家族基因mRNA表达的差异，探讨NFAT基因在肝癌发生发展中的作用。方法常规抽提30例患者肝癌组织与癌旁组织中的RNA，去除基因组DNA后反转录成cDNA，应用实时荧光定量聚合酶链反应（qPCR）检测标本中NFAT家族基因mRNA表达。结果NFATlmRNAratio癌组织为0．003034±0．002162，癌旁组织为0．010140±0．009742；NFAT2mRNAratio癌组织为0．000336±0．000272，癌旁组织0．002173±0．003380；NFAT3mRNAratio癌组织为0．002907±0．002436，癌旁组织为0．006427±0．004502；NFAT4mRNAratio癌组织为0．004198±0．004787，癌旁组织为0．012370±0．010890；NFAT5mRNAratio癌组织为0．079060±0．247600，癌旁组织为0．282100±0．686900；5种NFAT家族基因mRNA水平癌组织均较癌旁组织低（P〈0．01）。结论NFAT家族5种基因的mRNA水平痛组织较痛旁组织中均降低．可能与肝痛的发牛发展有关。Objective To investigate differential expression of nuclear factor of activated T cell （NFAT） gene family members mRNA in hepatocellular carcinoma （HCC） tissues and adjacent non-tumor- ous tissues of thirty patients, and to explorer the possible function in the process of oncogenesis and devel- opment of cancer. Methods The RNA was extracted from cancer tissue and adjacent non-tumorous tissue of thirty patients respectively, the genome DNA was removed and the RNA was reversely transcribed to cD- NA. NFAT gene family members mRNA was detected by applying Real time fluorescence quantitative poly- merase chain reaction （qPCR） in tissue samples. Results The mRNA ratio of NFAT1 in cancer tissue and adjacent non-tumorous tissue was 0. 003 034 -＋ 0. 002 162 and 0. 010 140 ＋ 0. 009 742 respectively. The mRNA ratio of NFAT2 in cancer tissue and adjacent non-tumorous tissue adjacent non-tumorous tissue adja- cent non-tumorous tissue was 0. 000 336 ＋ 0. 000 272 and 0. 002 173 ＋ 0. 003 380 respectively. The mR- NA ratio of NFAT3 in cancer tissue and adjacent non-tumorous tissue was 0. 002 907 ＋ 0. 002 436 and 0. 006 427 ＋ 0. 004 502 respectively. The mRNA ratio of NFAT4 in cancer tissue and adjacent non-tumor- ous tissue was 0. 004 198 ＋ 0. 004 787 and 0. 012 370 ~ 0. 010 890 respectively. The mRNA ratio of NFAT5 in cancer tissue and adjacent non-tumorous tissue was 0. 079 060 ~ 0. 247 600 and 0. 282 100 ~ 0. 686 900 respectively. The mRNA level of NFAT gene family＇ s five members all were lower in cancer tis- sue than in adjacent non-tumorous tissue （P 〈 0. O1 ）. Conclusion NFAT gene family＇ s five members mRNA expression is all down-regulated in cancer tissue as compared with that in adjacent non-tumorous tis- whiah max, h~ r^lAtarl ta nnaneana^i~ and nrnm＇agglnn nf llv^r eanear

关 键 词：癌 肝细胞 T细胞核因子 实时荧光定量PCR 

分 类 号：R735.7[医药卫生—肿瘤]