应用基因芯片筛选不同胶质瘤细胞相关基因  

Screening the glioma cells-associated genes by cDNA microarray

作　　者：张红[1] 楚胜华[1] 冯东福[1] 马延斌[1] 邱建华[1] 朱志安[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院神经外科,上海201900

出　　处：《中华实验外科杂志》2012年第2期253-255,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30901535）;上海交通大学医学院“新百人计划”资助项目（10XBR01）;上海交通大学医学院附属新华医院集团科研资助项目（10XHJT01）

摘　　要：目的应用高密度寡核苷酸（Oligo）基因芯片技术筛选不同胶质瘤细胞相关基因。方法提取星形胶质细胞瘤CHG-5细胞株（WHO分级Ⅱ级）、星形胶质细胞瘤U373细胞株（WHO分级Ⅲ级）、星形胶质细胞瘤SHG-44细胞株（WHO分级Ⅳ级）和神经胶质细胞U257细胞株的总RNA并逆转录为cDNA，其中cy5、cy3的dNTP分别掺人不同胶质瘤细胞和神经胶质细胞的cDNA，混合后杂交含22000个人类基因人类高密度Oligo基因芯片，经过洗片和扫描，获得荧光信号图像并用计算机分析，抽取4种差异表达基因用聚合酶链反应（PCR）验证它们不同胶质瘤细胞和神经胶质细胞中的差异表达。结果从22000条基因中筛选出差异表达基因242条，其中123条表达上调．119条表达下调，包括细胞凋亡、细胞周期蛋白、细胞骨架和运动蛋白等相关基因。结论基因芯片技术的胶质瘤基因表达谱分析能够高通量筛选胶质瘤相关基因，并高效对基因功能进行研究，有助于认识肿瘤发病机制。Objective To screen the glioma cells-associated genes by eDNA microarry. Methods The total RNAs were isolated from glioma cell hnes CHG-5, U373, SHG-44 and glial cells U257, and were reversely transfected to cDNAs and labeled with the fluorescent CyS-dNTP and Cy3-dNTP to prepare the hybridization probes. The fluorescent image was obtained and analyzed by computer. Results By applying the large-scale Oligo microarray, 242 differentially expressed genes in glioma were screened out among the 22 000target genes, comprising 123 up-regulated genes and 119 down-regulated genes respectively, including cell cycle related genes, cell apoptosis related genes and cell skeleton and cinetc-protein related genes. Conclusion The analysis of gene expression profile of glioma based on the large-scale Oligo microarray can realize high-throughput screening of the genes associated with the glioma, and it can help to rapidly explore the genes function. Further analysis of the obtained genes will help to understand the moleclllar mechanism of the zlioma.

关 键 词：胶质瘤 基因芯片 基因表达 

分 类 号：R739.41[医药卫生—肿瘤]