AFP、AFP-L3、GP73联合检测在肝癌诊断中的应用  

The application of combine detection of AFP、AFP-L3 and GP73 in the diagnosis of hepatocellular carcinoma

作　　者：王向臣[1] 曲建香[1] 鹿理友 于传亭[1] 林青 

机构地区：[1]山东省烟台市烟台山医院,山东烟台264000 [2]山东省烟台传染病医院,山东烟台264000

出　　处：《医学检验与临床》2011年第6期50-52,共3页Medical Laboratory Science and Clinics

摘　　要：目的 检测不同患者样本中甲胎蛋白（AFP）、甲胎蛋白异质体（AFP-L3）以及高尔基体蛋白73（GP73）的含量,研究AFP、AFP-L3、GP73在肝癌诊断中的应用.方法 收集肝癌患者样本、肝炎肝硬化样本以及正常健康人员样本,用电化学发光法检测AFP、AFP-L3及用ELISA法检测GP73含量.结果 1.本研究显示：AFP-L3%〉10%对肝癌诊断敏感度为89.1%;诊断特异度为94.4%[1];2.在101例肝癌标本中,GP73的阳性率为72.28%,AFP的阳性率37.62%,经过卡方检验,两者具有显著性差异（χ2=18.86,p〈0.01）;3.在所有非肝癌患者中,GP73的诊断特异性为93.44%;AFP的诊断特异性为86.48%.结论 AFP-L3、GP73 用于诊断肝癌效果明显优于AFP,可以显著提高对肝癌诊断的灵敏度和特异性,而且AFP-L3、GP73 相比AFP更不容易受到肝部炎症和其他部位肿瘤的影响.结合本人早期研究结果,将GP73、AFP-L3、AFP联合起来检测,能更好更快的对肝癌做出早期诊断.Objective To investigate the value of AFP, AFP - L3, and GP73 in the diagnosis of hepatoeellular carcinoma. Method AFP, AFP - L3 were quantified by ECL and GP73 was quantified by ELISA in the blood samples from patients with hepatocelluar carcinoma, hepatitis cirrhosis, and healthy controls. Results AFP - L3 〉 10% was valuable in the diagnosis of liver cancer with sensitivity of 89.1% and specificity of 94.4% ; The positive rates of GP73 and AFP in liver cancer patients were 72.28% and 37.62% respectively. They were statistically different （p 〈 0.01 ） ; The specificity of GP73 and AFP in non - cancer patients were 93.44 % and 86.48 % respectively. Conclusion AFP - L3, GP73 are more reliable than AFP in the diagnosis of hepatocellular carcinoma because they are not easy to be interfered by infections or tumors of other origins. The combination of AFP - L3 ,GP73, and AFP determination is recommended to be used clinically for early diagnosis of liver cancer.

关 键 词：肝癌 甲胎蛋白 甲胎蛋白异质体 高尔基体蛋白73 

分 类 号：R735.7[医药卫生—肿瘤]