电击死大鼠肝HSP60表达水平变化的法医学意义  

Medico-legal Significance of HSP-60 Changes in Rats＇ Liver after Electrocution

作　　者：王乔峰[1] 刘慧通[1] 谢亚男[1] 赵泽[1] 丁素真[1] 王振原[1] 

机构地区：[1]西安交通大学医学院,西安710061

出　　处：《现代检验医学杂志》2011年第6期24-27,共4页Journal of Modern Laboratory Medicine

基　　金：国家大学生创新性实验计划资助项目（编号：091069868）.

摘　　要：目的 探究无电流斑电击死后各阶段的大鼠肝脏热休克蛋白60（HSP60）表达情况.方法 制作无电流斑电击死动物模型,即大鼠按体重（5 ml/kg）腹腔注射20 ml/dl乌拉坦溶液,待麻醉生效后,将大鼠的左前肢与右后肢用生理盐水浸湿,外包以生理盐水浸泡过的湿纱布,施以110V交流电电击5 min致死,并设立对照组（空白组和死后电击组）,于死亡即刻,15,30 min,1,2,4 h取出大鼠肝右叶作切片,用HSP60抗体进行免疫组化染色,以图像平均灰度值作为HSP60表达情况指标作统计学分析.结果 电击死组肝细胞HSP60表达水平在15 min内表现为高表达,之后呈下降趋势.死后电击后15 min内表达较电击死组低,在30 min处表达超过电击死组,后下降,1 h处回升,2 h达高峰又回落,但其值始终高于电击死组.结论 死亡2 h内对肝脏HSP60表达水平的检测对鉴定无电流斑电击死及判断生前电击和死后电击有积极意义.Objective The objective of this paper is to observe the level-change of HSP-60（heat shock protein 60）in rats＇ liver after electrical injury and try to identify the forensic usage of the changes. Methods The animal model of electrical injury without electric marks was established and serum HSP-60 detection was done at 0,1/4,1/2,1,2,4h after electric shock（al- ternating current,voltage 110V） by immunohistochemistry. Results The liver cells which were electric shocked to deatb of HSP-60 in 15 minutes wss high performance expression,after which were declined. Electric group shock after death within 15 minutes after the death of a shock to express in 30 minutes,at low death group, expressed shock after more than decline, 1 h place picks up, 2 h of peak and dropped, butits value was always higher than the one which were electric shocked to death. Conclusion The changes of HSP-60 in rats＇liver within 2 h after death could be taken as a target in identifying the electrical injury without electric marks based the results mentioned above.

关 键 词：电击 热休克蛋白60 免疫组织化学法 大鼠 

分 类 号：R-332[医药卫生]