金磁微粒介导多克隆抗体纯化的最优化条件研究  

Optimal conditions of polyclonal antibody purification mediated by gold magnetic particles

作　　者：朱鸿林[1] 胡寅[1] 沈国清[1] 姜官鑫[1] 

机构地区：[1]上海交通大学农业与生物学院,农业部都市农业(南方)重点开放实验室,上海200240

出　　处：《食品工业科技》2012年第3期164-166,共3页Science and Technology of Food Industry

基　　金：上海市科委科技攻关项目(080391900300,08dz1900404);公益性行业(农业)科研专项(200903056);上海市科技兴农推广项目(沪农科推字2010第4-5号)

摘　　要：将牛血清白蛋白(BSA)包被在自制的金磁微粒表面,以金磁微粒与抗体比例、反应时间、反应温度为主要影响因素,依据均匀设计表,对盐酸克伦特罗(CL)多克隆抗体进行纯化,并对纯化前后抗体的效价以及抗体对CL的检测灵敏度进行了研究。结果表明,金磁微粒与抗体的比例以及两者的反应时间是影响抗体纯化的主要因素;纯化后多克隆抗体中抗BSA抗体明显下降,而抗CL抗体基本保持不变;对比纯化前后CL的检测曲线,纯化后的最低检测限(LOD)和半抑制浓度(IC50)分别下降了6.90%和24.37%。Gold magnetic particles coated with bovine serum albumin（BSA） were used to purify the polyclonal antibody of clenbuterol（CL）.The proportion of polyclonal antibody and gold magnetic particles,reaction time,temperature were designed to be the influencing factors,and the results showed that the proportion and the reaction time were the main factors.Compared with the polyclonal antibody without purifying,the BSA-antibody was decreased obviously after purifying;the limit of detection（LOD） and half-inhibitory concentration（IC50） were decreased 6.9% and 24.37%.

关 键 词：金磁微粒 盐酸克伦特罗 抗体纯化 最优化 

分 类 号：Q511[生物学—生物化学]