豆类中4种嘌呤的高效液相色谱测定法  

Determination of purines in beans by high performance liquid chromatography

作　　者：刘桂英[1] 张加玲[1] 王婷[1] 

机构地区：[1]山西医科大学公共卫生学院卫生化学教研室,太原030001

出　　处：《中国卫生检验杂志》2012年第1期11-13,共3页Chinese Journal of Health Laboratory Technology

基　　金：山西省自然科学基金(2010011054-1)

摘　　要：目的:建立豆类中嘌呤含量测定的高效液相色谱法(HPLC)。方法:采用10%(v/v)高氯酸溶液在100℃水浴中水解样品60 min,样品液调节pH值,过滤后,进行色谱分析。色谱柱为Agilent ZORBAX Eclipse XDB-C18(250×4.6 mm,5μm),柱温25℃,流动相为0.007 mol/L KH2PO4(pH=4.0),流速1.0 ml/min,紫外检测器254 nm处检测,进样量为20μl。结果:在0.1 mg/L～25 mg/L的浓度范围内,各嘌呤的响应峰面积与浓度呈良好线性相关,r>0.9997,相对标准偏差<8.74%,样品加标回收率为85.9%～104.3%。结论:该方法测定豆类中嘌呤含量,具有简便快速、结果准确可靠、重现性好等优点。不同豆类的嘌呤总含量在58.2～158.1 mg/100 g之间。Objective：To establish a method for determination of purines in beans by high performance liquid chromatography （HPLC）. Methods： The beans were hydrolyzed with 10% （v/v）perchloric acid at 100℃ for 60 min. After acidity regulation and filtration, sample solution was injected with ultraviolet - visible detector at 25℃ with detection wavelength of 254 nm. The column was Agilent ZORBAX Eclipse XDB - C18（250 ×4.6 mm,5 μm） and mobile phase was 0.007 mol/L KH2PO4（pH =4.0） with a flow rate of 1.0 ml/min. The injection volume was 20 μl. Results： The peak area of purines and concentration achieved good linear relation in the range of 0.1 mg/L to 25 mg/L with correlation coefficient more than 0.9997. The relative standard deviation was less than 8.74% and the recovery was in the range of 85.9% - 104.3%. Conclusion： The method is simple,rapid, accurate and reproducible to determinate purines in beans. The total content of purines in beans is in the range of 58.2 158.1 mg/100 g.

关 键 词：嘌呤 高效液相色谱法 豆类 

分 类 号：O657.72[理学—分析化学]