PAX6基因及其下游促分化基因在视网膜母细胞瘤分化过程中的作用  

The role of PAX6 and its downstream gene on differentiation of human retinoblastoma cells

作　　者：申令[1] 史季桐[1] 李彬[1] 赵博文[1] 白淑玮[1] 张浩[1] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科学与视觉科学重点实验室北京市眼科研究所,100005

出　　处：《眼科》2011年第6期408-411,共4页Ophthalmology in China

基　　金：国家自然科学基金资助项目(30772377)

摘　　要：目的检测在视网膜母细胞瘤(retinoblastoma,RB)中,眼发育调控基因PAX6及其下游促分化基因MATH5和BRN3b在蛋白水平的表达情况,从而探讨PAX6基因在RB分化过程中的作用。设计病例对照研究。研究对象新鲜RB组织10例,正常视网膜组织10例。方法实验组:RB组织;对照组:正常视网膜组织。采用WesternBlot法检测新鲜视网膜母细胞瘤组织和正常视网膜组织中Pax6、Math5、Brn3b蛋白质水平的表达情况并作比较。主要指标Pax6、Math5、Brn3b蛋白质的表达水平。结果本研究中RB组织和正常视网膜组织中均可检测出Pax6、Math5、Brn3b蛋白的表达。与正常视网膜组织相比较,RB组织中Pax6、Math5、Brn3b蛋白的表达水平增高(P均<0.001);Math5与Pax6蛋白表达具有线性相关性(r=0.949,P<0.001),Brn3b与Math5蛋白表达具有线性相关性(r=0.927,P<0.001)。结论在RB组织中,PAX6基因上调下游促分化基因MATH5和BRN3b表达水平,促进RB组织的分化。Objective To detect the protein expression of PAX6,MATH5 and BRN3b in retinoblastoma（RB） and normal retinal tissue.Design Case-control study.Participants Ten cases of fresh RB solid tumors and ten cases of normal retinal tissue.Methods Objectives were collected and studied by WesternBlot to compare the protein expression of Pax6,Math5 and Brn3b in fresh RB solid tumors with those in normal retinal tissue.Main Outcome Measrues The protein expression of Pax6,Math5 and Brn3b.Results The protein expresion of Pax6,Math5 and Brn3b were positive in both groups.Pax6,Math5 and Brn3b protein expression in RB tumors increased significantly compared with those in normal retinal tissue（P all0.001）.The protein expression of Math5 positively correlated with the Pax6 expression（r=0.949,P0.001）,and the protein expression of Brn3b positively correlated with the Math5 expression（r=0.927,P0.001）.Conclusion PAX6 gene has a role in differentiation of RB cells through up-regulated the protein expression of MATH5 and BRN36 gene.

关 键 词：视网膜母细胞瘤 分化 PAX6 Math5 Brn3b 

分 类 号：R739.7[医药卫生—肿瘤]