液相色谱—串联质谱法测定牛肉中磺胺类抗生素  

Determination of Sulfonamide in Beef by High Performance Liquid Chromatography-tandem Mass Spectrometry

作　　者：李谦 刘正华 李拥军 

机构地区：[1]广东省中山市小榄镇农业服务中心,广东中山528415 [2]中山市农产品质量监督检验所

出　　处：《现代农业科技》2012年第2期345-346,共2页Modern Agricultural Science and Technology

基　　金：国家自然科学基金项目(30670380);农业部生态农业重点开放实验室开放课题(2009k07);农业科技攻关项目(20114A199)

摘　　要：采用液相色谱一串联质谱法检测牛肉中的7种磺胺类药物残留。样品经乙腈提取，正己烷脱脂净化，液相色谱一串联质谱法检测。结果表明：7种磺胺类药物均具有良好的线性关系，相关系数不低于0．998，高、中、低3档加标回收率为92．5％．100．6％，相对标准偏差为2．0％-4．5％，检出限为1．0-4．5μg／L。该方法具有检测结果准确可靠、简便快速等优点。A HPLC-MS/MS method for determination of 7 sulfonamides residues in beef was made in the paper.The sample was extracted with acetonitrile and anhydrous sodium sulfate,purified and defatted with n-hexane,then detected by high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）.The results showed that calibration curves for 7 sulfonamides residues all had good linear relationships with correlation coefficients not less than 0.998. The spiked recoveries at three concentrations were ranged from 92.5% to 100.6% ,with relative standard deviations of 2.0%.4.5%.The limits of detection were in the range of 1.0-4.5 μg/L.It was an effective method which fitted for the sulfonamides residues analysis and had high accuracy, precision and sensitivity.

关 键 词：牛肉 磺胺类药物 残留 液相色谱--串联质谱法 

分 类 号：O657.75[理学—分析化学] O657.63[理学—化学]