姜黄素对多巴胺能细胞的保护作用研究  

Protective effect of curcumin against rotenone-induced toxicity in NGF-differentiated PC12 cells

作　　者：胡丹[1] 汪宁[1] 张振涛[1] 张兆辉[1] 

机构地区：[1]武汉大学人民医院神经内科,430060

出　　处：《卒中与神经疾病》2011年第6期326-328,347,共4页Stroke and Nervous Diseases

摘　　要：目的探讨姜黄素对多巴胺能细胞的保护作用及机制。方法采用神经毒素鱼藤酮(1μmol/L)处理经神经生长因子(NGF)(50 ng/ml×7)诱导分化过的PC12细胞,并在处理前6 h加入1μmol/L的姜黄素进行干预,MTT法检测细胞活力,荧光分光光度法检测细胞内活性氧水平,比色法检测还原型谷胱甘肽(GSH)含量。结果 NGF诱导后的PC12细胞经1μmol/L鱼藤酮处理24 h后,细胞活力下降至对照组的57.1%,细胞内活性氧水平为对照组的189%,而GSH水平显著下降(P<0.05);姜黄素干预后,与鱼藤酮组相比,细胞活力和GSH含量显著提高,活性氧水平显著降低(P<0.05)。结论姜黄素对多巴胺细胞具有保护作用,其机制与抗氧化活性有关。To investigate the protective effect of curcumin on NGFdifferentiated PC12 cells in rotenoneinduced cell injury. Methods PC12 cells were differentiated by NGF at 50 ng/ml for 7 days, and then treated with 1 /μmol/L of rotenone for 24 h. Additionally, curcumin （1μmol/L） was added 6 h before rotenone treatment. Cell viability and reactive oxygen species （ROS） were determined with MTT method and fluorescent spectrophotometer assay, respectively. Glutathione assay kit was used to assess the level of intracellular glutathione. Results After rotenone treatment, the cell viability of PC12 cells was down to 57. 1% of control group. ROS level increased to 189% of control. Rotenone treatment also decreased the level of intracellular GSH （P〈0. 05）. Pretreatment with curcumin led to increased cell viability, decreased ROS formation and elevated GSH levels when compared with rotenone group （P〈0. 05）. Conclusions Curcumin had the property of protecting NGFdifferentiated PC12 cells against rotenone and the mechanism was related with its antioxidant ability.

关 键 词：姜黄素 鱼藤酮 帕金森病 多巴胺能细胞 

分 类 号：R741[医药卫生—神经病学与精神病学]