光动力疗法对HepG2肝癌细胞增殖的抑制作用  

Inhibition of photodynamic therapy on proliferation of HepG2 hepatoma cell in vitro

作　　者：曲娟[1] 陆伟[2] 

机构地区：[1]天津市南开医院消化内科,300100 [2]天津市传染病医院

出　　处：《肝脏》2011年第6期467-470,共4页Chinese Hepatology

摘　　要：目的探讨photosan-光动力疗法(PDT)在不同实验条件下对人HepG2肝癌细胞增殖的抑制作用及其作用机制。方法应用体外培养的人HepG2肝癌细胞,以photosan为光敏剂、半导体激光器(波长630 nm)为光源进行激光照射,在不同浓度光敏剂经不同剂量的光照后,用MTT法测定PDT后HepG2肝癌细胞的残存率,并用流式细胞分析检测PDT对肝癌细胞周期及凋亡的影响。结果 PDT对肝癌细胞株有明显的抑制作用,在一定范围条件下(光剂量小于12 J/cm^2),随着光敏剂浓度的增加和激光剂量的增大,肝癌细胞的残存率明显下降。同时发现在相同的光剂量和光敏剂浓度、不同的激光强度下(30 mW/cm^2、50 mW/cm^2),疗效亦有差异。流式细胞分析显示,PDT后HepG2肝癌细胞复制周期被阻滞于G1期,且出现了明显的凋亡。结论 PDT对体外培养的人肝癌细胞具有明确的抑制作用,这种抑制作用可能由于PDT后正常的肝癌细胞复制周期被阻断,从而导致细胞凋亡。Objective The experiment is to observe the inhibitory effect of PDT for hepatoma cell under different experiment conditions and to primarily observe the mechanism of PDT.Methods HepG2 human hepatoma cells were routinely cultured.The cell viability was determined by MTT assay after being treated by PDT with different concentration photosan and different light doses.And then the effect of PDT in the cell cycle and cell apoptosis were detected by flow cytometer（FCM）.Results MTT test showed that the inhibitory effect of PDT for hepatoma cell was significant.The survival descended obviously（P 0.05） along with the increased light dose and the photosan concentration in some condition.When treated with the light dose over 12 J/cm^2,the descending of the survival was slow even no further change（P0.05） occurs.Besides,the light intensity of 30 mW/cm^2 was superior to 50 mW/cm^2,when the photosan concentration and the light dose were the same.FCM test showed that the cell cycle was blocked to G1 phase after PDT and the cell apoptosis was induced by PDT.Conclusion PDT could significant inhibit the growth of hepatoma cell which might be induced by the arrest of cell cycle and thus increase cell apoptosis.

关 键 词：光动力疗法 残存率 细胞周期 细胞凋亡 HEPG2肝癌细胞 

分 类 号：R735.7[医药卫生—肿瘤]