新型交联剂三羟甲基磷固定化脂肪酶的研究  

Lipase Immobilization with a New Crosslinking Reagent Tris(hydroxymethyl)phosphine

作　　者：梁欣欣[1] 魏东[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《现代食品科技》2012年第1期47-51,共5页Modern Food Science and Technology

基　　金：国家海洋局海洋可再生能源专项资金项目(GHME2011SW04)

摘　　要：本试验研究了一种新型交联剂三羟甲基磷(THP)用于以纯棉浴巾载体、聚乙烯亚胺(PEI)为吸附剂的固定化脂肪酶制备新方法。通过单因子实验获得了固定化条件为:0.2 g浴巾使用0.5 mLPEI(浓度3.37 mg/mL,pH 7.0)、2 mLTHP(浓度为0.10%,V/V)、固定化温度为40℃、交联时间为20 min。制备的固定化脂肪酶催化正丁酸乙烯酯与戊醇酯交换反应,1 h底物转化率达92.1%,酶比活力达到15.80 U/g固定化酶。与戊二醛(GA)交联法制备的固定化酶相比,本固定化酶具有良好的重复使用稳定性、温度稳定性和高比活力,本试验采用的方法是一种低成本、实用性强的脂肪酶固定化新方法。In the present work, tris（hydroxymethyl）phosphine （THP） was used as a new crosslinking reagent in lipase immobilization on cotton towel with adsorbent polyethylcnimine （PEI）. The optimal conditions of immobilization by single factors test were obtained as follows： 0.5 mL of PEI （3.37 mg/mL, pH 7.0） for each 0.2 g cotton rowe, 2ml of THP [0.10% （V/V）], immobilization temperature 40 ＂C and crosslinking reaction time 20 min. THP-immobilized lipase can catalyze intcrestcrification of vinyl butyric acid and pcntanol. The conversion rate in lh achieved 92.1%, and the activity of immobilized lipase was 15.80 U/g. Compared with the GA-irnmobilized lipase, THP-immobilized lipase had higher reusability, thermal stability and activity, indicating this is a low cost and practical newly method for lipase immobilization.

关 键 词：脂肪酶 固定化 交联剂 三羟甲基磷 戊二醛 

分 类 号：Q814.2[生物学—生物工程]