丹参注射液联合5-氟尿嘧啶对小鼠Lewis肺癌细胞凋亡的影响  

Study on Salviae Miltiorrhizae Injection in Combination with 5-Fu for the Apoptosis of Lewis Lung Tumor Mice

作　　者：冯俭[1] 谢萍[1] 秦银花[2] 孙川[2] 张煜华[2] 张梦云[2] 杨张婧[2] 莫小宇[2] 王倩[2] 余晓颖[2] 

机构地区：[1]成都中医药大学附属医院 [2]成都中医药大学

出　　处：《中医杂志》2012年第3期238-240,258,共4页Journal of Traditional Chinese Medicine

基　　金：四川省科技厅应用技术研究与开发项目(2008JY0008)

摘　　要：目的 探讨丹参注射液联合5-氟尿嘧啶(5-Fu)对小鼠Lewis肺癌细胞凋亡的作用。方法 将60只Lewis肺癌荷瘤C57BL/6小鼠随机分为模型组,丹参高、低剂量组(丹高组、丹低组),5-Fu组,丹参高、低剂量联合5-Fu组(丹低+5-Fu组、丹高+5-Fu组),每组10只。各组于接种第2日起每天腹腔注射给药0.2ml/只,5-Fu组连续给药5天,联合组先给5-Fu及丹参注射液混合液5天,后继续给予丹参注射液5天。其余各组均连续给药10天。末次给药24h后摘眼球取血再脱颈椎处死,观察血常规、瘤体重量、肺癌细胞凋亡指数、主要脏器转移灶数目及病理改变。结果 丹参低剂量组和丹参高剂量组瘤体重量较模型组降低(P<0.05),5-Fu组、丹参低剂量+5-Fu、高剂量+5-Fu组较模型组瘤体重量明显减轻(P<0.01)。丹参高剂量组、5-Fu组、丹参低剂量+5-Fu组及丹参高剂量+5-Fu组瘤体细胞凋亡指数与模型组比均增高(P<0.05)。结论 丹参注射液能够增强5-Fu对肿瘤的抑制,有增强小鼠Lewis肺癌细胞凋亡作用的趋势。Objective To observe the effect of Danshen （Radix Salviae Miltiorrhizae） Injection used together with 5-fluorouracil （5-Fu） on the apoptosis of Lewis lung tumor mice. Methods Sixty C57BL/6 mice with Lewis lung tumors were randomized into model control group, Danshen high-dose group and low-dose group, 5-Fu group, Danshen high-dose with 5-Fu group, and Danshen low-dose with 5-Fu group, with 10 in each. All mice were intraperitoneally injected 0.2ml everyday from the second day of inoculation. The 5-Fu group was injected for 5 days in succession, the united 5-Fu groups were given the mixture of 5-Fu and Danshen injection first for 5 days and then Danshen injection in continuation for 5 days, and the other groups were all given medicine for 10 days in succession. Twenty-four hours after the last administration, the eyeballs of mice were removed for collecting the orbital blood, and then were sacrificed. The routine blood test, weight of tumors, apoptosis index, the number of main organ metastases and the pathological changes were observed. Results Compared with the model control group, the weight of tumors of the Danshen low-dose group and high-dose group was decreased （P〈0.05）, the weight of tumors of the 5-Fu group, the Danshen low-dose with 5-Fu group and Danshen high-dose with 5-Fu group were significantly decreased （P〈0.01）; apoptosis index of tumor cells of the Danshen high-dose group, 5-Fu group, Danshen low-dose with 5-Fu group, and Danshen high-dose with 5-Fu group were all increased （P〈0.05）. Conclusion Danshen Injection is effective in enhancing the inhibition of 5-Fu for tumor and promoting the apoptosis of Lewis lung tumor mice.

关 键 词：丹参注射液 5-氟尿嘧啶 抑瘤率 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]