海洋来源真菌Hypocrea virens的次级代谢产物研究  

Studies on the secondary metabolites from the marine-derived fungus Hypocrea virens

作　　者：刘涛[1] 李占林[2] 王宇[2] 田黎[3,4] 裴月湖[2] 华会明[2] 

机构地区：[1]中国医科大学药学院,辽宁沈阳110001 [2]沈阳药科大学创新药物研究与设计教育部重点实验室,辽宁沈阳110016 [3]国家海洋局第一海洋研究所,山东青岛266061 [4]青岛科技大学,山东青岛266042

出　　处：《沈阳药科大学学报》2012年第2期93-97,共5页Journal of Shenyang Pharmaceutical University

基　　金：国家863项目资助项目(2007AA09Z413;2007AA09Z435);国家自然科学基金资助项目(20872094)

摘　　要：目的研究海洋来源真菌Hypocrea virens发酵液的化学成分。方法采用硅胶柱色谱、Sepha-dex LH-20凝胶柱色谱、开放ODS柱色谱以及HPLC等方法进行分离纯化,通过理化性质和波谱数据鉴定化合物的结构。结果分离得到10个化合物,分别鉴定为bisdethiobis-(methylthio)glio-toxin(1)、胶霉毒素(gliotoxin,2)、5-羟基-3-羟甲基-2-甲基-7-甲氧基色原酮(5-hydroxy-3-hydroxymethyl-2-methyl-7-methoxychromone,3)、6-甲基苯-1,2,4-三醇(6-methyl-benzene-1,2,4-triol,4)、3β-羟基-胆甾-5-烯(3β-hydroxy-cholesta-5-ene,5)、3-异丁基-8-羟基吡咯并哌嗪-2,5-二酮(3-isobutyl-8-hydroxypyrrolopiperazine-2,5-dione,6)、3-苄基哌嗪-2,5-二酮(3-benzylpiperazine-2,5-dione,7)、3-苄基-8-羟基吡咯并哌嗪-2,5-二酮(3-benzyl-8-hydroxypyrrolopiper-azine-2,5-dione,8)、3S*,4R*-二羟基-3-甲基戊烷-2-酮(3S*,4R*-dihydroxy-3-methylpentan-2-one,9)、3R*,4R*-二羟基-3-甲基戊烷-2-酮(3R*,4R*-dihydroxy-3-methylpentan-2-one,10)。结论化合物1、3、4、8～10均为首次从Hypocrea属真菌中分离得到,化合物5为首次从真菌Hypocrea virens中分离得到。Objective To study chemical constituents from the fermentation broth of marine-derived fungus Hypocrea virens. Methods The compounds were isolated and purified by silica gel, Sephadex L H-20, open ODS column chromatography and HPLC, and their structures were elucidated on the basis of spectral data and physicochemical properties. Results Ten compounds were isolated from the fermentation broth of mafine-derived fungus Hypocrea virens. Their structures were identified as bisdethiobis-（methylthio） gliotoxin （ 1 ）, gliotoxin （ 2 ）, 5-hydroxy-3 -hydroxymethyl-2-methyl-7-methoxychromone （ 3 ）, 6-methylbenzene-1,2,4- triol （ 4 ）, 3β-hydroxy-cholesta-5-ene （ 5 ）, 3-isobutyl-8-hydroxypyrrolopiperazine-2,5-dione （ 6 ）, 3-benzyl pi- perazine-2,5-dione （ 7 ）, 3-benzy1-8-hydroxypyrrolopiperazine-2,5-dione （ 8 ）, 3 S ＊ , 4R ＊ -dihydroxy-3 -methyl- pentan-2-one （ 9 ）, 3R＊ , 4R ＊ -ihydroxy-3-methylpentan-2-one （ 10 ）. Conclusions Compounds 1,3,4,8 - 10 are isolated from the Hypocrea genus of marine-derived fungus for the first time, and compound 5 is isolated from the marine-defived fungus Hypocrea virens for the first time.

关 键 词：海洋来源真菌 HYPOCREA virens 次级代谢产物 化学成分 结构鉴定 

分 类 号：R914[医药卫生—药物化学] R931.77[医药卫生—药学]