SurvivinsiRNA对人肺腺癌细胞株AGZY增殖及凋亡的影响，  

Study about survivin siRNA effects of proliferation and apoptosis in human lung adenocarcinoma cell line AGZY

作　　者：刘静蕾[1] 姜季[2] 赵红丽[1] 李欣[1] 浦海宏[1] 王艳[1] 

机构地区：[1]哈尔滨医科大学附属第三医院肿瘤内科,150081 [2]哈尔滨医科大学附属第三医院同位素科,150081

出　　处：《国际遗传学杂志》2012年第1期1-5,共5页International Journal of Genetics

基　　金：基金项目：黑龙江省自然科学基金（D200849）

摘　　要：目的探讨靶向survivin基因特异性siRNA对人肺腺癌细胞株AGZY的凋亡、增殖影响以及对其相关机制的探讨。方法采用RNAi技术靶向干扰AGZY细胞survivin基因的表达，分空白组、转染试剂组、阴性对照组、转染组，用RT—PCR检测各组survivin基因的表达，绘制细胞生长曲线，Hoechst染色检测各组细胞凋亡情况，Western印迹检测各组蛋白survivin、caspase-3、caspase-8、cyclinB1、cyclinD1的变化。结果Survivin特异性siRNA可在mRNA及蛋白水平有效下调survivin的表达，转染组与其他3组比较差异有统计学意义（F=1785．25，F=38．67，P〈0．05）；survivin表达抑制后，与其他3组比较，转染组AGZY细胞数明显减少（F=14999．46，P〈0．05），且转染组细胞出现典型的凋亡改变；与其他3组相比较，survivin特异性siRNA转染组cyclinB1、cyclinD1表达下调（F=109．674，F=313．102，P〈0．05），caspase-3、caspase．8表达上调（F=101．60，F=782．504，P〈0．05）。结论Survivin基因特异性siRNA可以有效下调survivin的表达；survivin表达下降可促进细胞凋亡，抑制细胞增殖；细胞增殖受抑制可能与cyclinB1、cyclinD1表达下降有关，细胞凋亡增加可能与caspase-3、caspase-8表达上调有关。Objective To investigate the effects of survivin-specific siRNA on the proliferation and apoptosis of human lung adenocarcinoma cell line AGZY, and to explore their mechanisms. Methods Survivin-specific siRNA was tranfected into AGZY cells. Four＇ groups were included： negative control group, DharmaFECT-treated group, nonsense siRNA-treated group, survivin specific siRNA-treated group. The expression of survivin mRNA levels was determined using RT-PCR. Cell growth was studied. The apoptosis was analyzed by Hoechst 33258 staining method. The protein expression levels was deter- mined using Western blotting analyses. Results Campared with other three groups, survivin-specific siRNA could efficiently down-regulate the survivin expression at mRNA and protein levels （F = 1785.25 , F = 38.67, P 〈 0.05 ）. After transfection, the cell proliferation decreased significantly com- pared with other three groups（ F = 14999.46 ,P 〈 0.05 ）. The typical changes associated with apopto-sis was observed in the transfected group. Compared with other three groups, in transfected group, the ex- pression of caspase-3 and caspase-8 was upre gulated （ F -- 101.60, F = 782. 504, P 〈 0.05 ） , and the expression of cyclinB1 and cyclinDl was downregulated （F = 109. 674, F = 313. 102, P 〈 0.05 ）. Conclusions Survivin-specific siRNA can supresses the expression of survivin, increase apoptosis, and inhibit cells proliferation. It may have relationship with the upregulation of caspase-3 and easpase-8, and the downregulation of cyclinB1 and cyclinD 1.

关 键 词：人肺腺癌 SURVIVIN SIRNA 凋亡 裸鼠 

分 类 号：R734.2[医药卫生—肿瘤]