Biochemical analysis of granzyme H and elucidation of its structurally important residues involved in substrate and inhibitor binding  

作　　者：Naheed Z Razwi Rukhshan Khurshid Mahjabeen Saleem Sabiha Karim Saeed Ahmad Nagra Asmat Salim 

机构地区：[1]Department of Biochemistry,CMH Lahore Medical College,Lahore,Pakistan [2]Fatima Jinnah Medical College,Lahore,Pakistan [3]Institute of Biochemistry and Biotechnology,University of the Punjab,Lahore,Pakistan [4]College of Pharmacy,University of the Punjab,Lahore [5]Institute of Chemistry,University of the Punjab,Lahore,Pakistan [6]Dr.Panjwani Center for Molecular Medicine and DrugResearch,International Center for Chemical and Biological Sciences,Karachi University,Pakistan

出　　处：《Journal of Chinese Pharmaceutical Sciences》2012年第1期33-39,共7页中国药学（英文版）

摘　　要：Apoptotic cell death plays an important role in the maintenance of the normal physiological state and in the pathogenesis of diseases.Granule exocytosis is the main pathway for the immune elimination of virus-infected cells and tumor cells by cytotoxic T lymphocytes and natural killer cells.In recent study,we have investigated the level of granzyme H in patients with breast cancer and in control subjects using enzymatic method.Our study also included the prediction of different sites of granzyme H that play a role in substrate and inhibitor recognition in apoptosis process by using 3D structural model of the enzyme.The research described the possible post-translational modification sites that may help the enzyme in immune elimination of tumor cells.Our study shows that the level of granzyme H was reduced in patients when compared to normal control subjects.There are a number of amino acids that function as substrate recognition sites in granzyme H.However,inhibitors may inhibit their activity and affect the process of autolysis.Apoptotic cell death plays an important role in the maintenance of the normal physiological state and in the pathogenesis of diseases. Granule exocytosis is the main pathway for the immune elimination of vires-infected cells and tumor cells by cytotoxic T lymphocytes and natural killer cells. In recent study, we have investigated the level of granzyme H in patients with breast cancer and in control subjects using enzymatic method. Our study also included the prediction of different sites of granzyme H that play a role in substrate and inhibitor recognition in apoptosis process by using 3D structural model of the enzyme. The research described the possible post-translational modification sites that may help the enzyme in immune elimination of tumor cells. Our study shows that the level of granzyme H was reduced in patients when compared to normal control subjects. There are a number of amino acids that function as substrate recognition sites in granzyme H. However, inhibitors may inhibit their activity and affect the process of autolysis.

关 键 词：AUTOLYSIS Granzyme H Apoptosis Post translational modification 

分 类 号：R730.2[医药卫生—肿瘤]