14-3-3σ和Mdm2蛋白在浸润性乳腺癌中的表达及意义  

Clinical significance of 14-3-3σ and Mdm2 protein expression in infiltrating breast cancer

作　　者：刘廷[1] 齐凤杰[1] 赵树鹏[2] 姚萍[1] 

机构地区：[1]辽宁医学院病理教研室,辽宁锦州121001 [2]辽宁医学院附属第一医院普外科,辽宁锦州121001

出　　处：《广东医学》2012年第2期192-195,共4页Guangdong Medical Journal

基　　金：辽宁省科学技术计划项目(编号:2010225034)

摘　　要：目的研究浸润性乳腺癌组织中14-3-3σ和Mdm2蛋白的表达情况及其与临床病理参数之间的关系。方法采用免疫组化PV-9000两步法检测浸润性乳腺癌73例、乳腺导管原位癌20例、乳腺导管上皮不典型增生20例、导管内乳头状瘤10例、乳腺腺病10例和癌旁正常乳腺组织12例中14-3-3σ和Mdm2蛋白的表达情况。结果 73例浸润性乳腺癌组织中14-3-3σ的阳性表达率为20.5%,20例乳腺导管原位癌为40.0%,20例导管上皮不典型增生为65.0%,10例导管内乳头状瘤为70.0%,10例乳腺腺病为80.0%和12例癌旁正常组织为83.3%,其中浸润性乳腺癌明显低于乳腺癌旁正常组织(P<0.05);14-3-3σ的表达在TNM分期、腋窝淋巴结转移、HER-2受体及ER受体等组间差异均有统计学意义(P<0.05),其他临床特征组间差异均无统计学意义(P>0.05);Mdm2蛋白在浸润性乳腺癌、乳腺导管原位癌、导管上皮不典型增生、导管内乳头状瘤、腺病及癌旁正常组织中的表达阳性率分别为61.6%(45/73)、55.0%(11/20)、45.0%(9/20)、40.0%(4/10)、30.0%(3/10)30%和16.7%(2/12),其中浸润性乳腺癌明显高于癌旁正常组织(P<0.05);Mdm2的表达与腋窝淋巴结转移有关(P<0.05),而与其他临床病理参数无关(P>0.05);14-3-3σ和Mdm2蛋白的表达在浸润性乳腺癌中呈负相关(rs=-0.458,P=0.000)。结论 14-3-3σ和Mdm2蛋白在浸润性乳腺癌中异常表达,说明其可能在乳腺癌的发生发展中起重要作用。Objective To investigate the expression of 14-3-3σ and Mdm2 proteins in invasive breast cancer and its significance in prognosis.Methods The expression of 14-3-3σ and Mdm2 was assessed in 73 infiltrating breast cancers,20 ductal carcinoma in situ,20 ductal epithelial hyperplasia of mammary gland,10 intraductal papilloma,10 adenosis of breast and 12 adjacent normal breast tissue samples by immunohistochemistry PV two-step method.Results The positive rates of 14-3-3σ in infiltrating breast cancer,ductal carcinoma in situsignificantly,ductal epithelial hyperplasia of mammary gland,intraductal papiloma of breast,adenosis of breast and adjacent normal breast tissue were 20.5%,40.0%,65.0%,70.0%,80.0% and 83.3%,respectively,with significant difference between infiltrating breast cancer and adjacent normal tissue（P0.05）.The expression of 14-3-3σ was significantly correlated with TNM stage,axillary lymph node metastasis,recurrence,HER-2 and ER expression（P0.05）,but with no significant correlation with other clinical features（P0.05）.The positive rates of Mdm2 in infiltrating breast cancer,ductal carcinoma in situ,ductal epithelial hyperplasia of mammary gland,intraductal papiloma of breast,adenosis of breast and adjacent normal breast tissue were 61.6%,55.0%,45.0%,40.0%,30.0% and 16.7%,respectively,with significant diffe-rence between infiltrating breast cancer and adjacent normal breast tissue（P0.05）.The expression of Mdm2 was significantly correlated with axillary lymph node metastasis（P0.05）,but with no significant correlation with the other clinical features（P0.05）.There was significantly negative correlation between the expression of 14-3-3σ and Mdm2 in infiltrating breast cancer（rs=-0.458,P=0.000）.Conclusion The altered expression of 14-3-3σ and Mdm2 proteins in infiltrating breast cancer plays important role in breast cancer pathogenesis.

关 键 词：浸润性乳腺癌 14-3-3σ MDM2 免疫组化 

分 类 号：R737.9[医药卫生—肿瘤]