两株分解除草剂丁·苄的双效菌株筛选、鉴定及其降解特性研究  

A Study on Screening,Identification and Degradation Characteristics of Two Strains with Double Effects on Butachlor and Bensulfuron Methyl

作　　者：刘军[1] 霍光华[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《江西农业大学学报》2011年第6期1212-1218,共7页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家科技支撑项目(2006BAD05B09);江西省研究生创新基金项目(YC08A055)

摘　　要：为了获得能同时分解丁草胺和苄嘧磺隆的菌株,从连续施用丁.苄3年以上的水稻田土壤中分离筛选出15株能以丁草胺和苄嘧磺隆为唯一碳源的菌株,进一步复筛获得了两株菌Dx9、Dx12。通过ITS序列分析确定Dx9与青霉属的Penicillium expansum Y2-08(GU134896),Dx12与曲霉属的Aspergillus fumigatus(FM999061)均有99%同源性。在底物浓度为100 mg/L(20%丁.苄500 mg/L),pH 7.0,培养温度为35℃,摇床转速为180 r/min的条件下,接种1.0 mL(107 cfu/mL)培养72 h,Dx9对丁草胺和苄嘧磺隆的降解率分别为87.3%和95.6%,Dx12对丁草胺和苄嘧磺隆的降解率分别为92.1%和88.5%。由此可见,该两菌株对除草剂丁.苄污染的土壤具有较好地生物修复作用。Two strains of fungi Dx9 and Dx12 capable of simultaneously degrading butachlor and bensulfuron methyl were obtained from primarily screened fifteen strains which only degradate butachlor or bensulfuron methyl from paddy field soil which was applied with butachlor and bensulfuron methyl for over three years.Based on the phylogenetic analysis of ITS sequence,the strains Dx9 and Dx12 were identified as 99% homologous with Penicillium expansum Y2-08（GU134896） and Aspergillus fumigatus（FM999061）respectively.The degradation rates of strain Dx9 on butachlor and bensulfuron methyl were 87.3% and 95.6%,while the rates of strain Dx12 were 92.1% and 88.5% at the concentration of 100 mg/L（20% butachlor and bensulfuron methyl 500 mg/L） and under the condition of 30 ℃,pH 7.0,180 r/min,inoculation 1×107 cfu/mL and incubation 72 h.Judging from this,the strains Dx9 and Dx12 are of good bioremediation function for soil polluted by herbicides of butachlor and bensulfuron methyl.

关 键 词：双效分解菌 丁草胺 苄嘧磺隆 降解特性 

分 类 号：X172[环境科学与工程—环境科学]