球孢白僵菌BbⅢ22固态发酵条件的优化  

Optimization of Solid-state Fermentation Conditions for Beauveria bassiana BbⅢ22

作　　者：邓小军[1] 周国英[1] 刘君昂[1] 闫瑞坤[1] 陈春林[1] 杨枝林[1] 

机构地区：[1]中南林业科技大学林业生物技术湖南省重点实验室,湖南长沙410004

出　　处：《江西农业大学学报》2011年第6期1228-1235,共8页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家林业公益性行业科研专项(201004003)

摘　　要：对球孢白僵菌BbⅢ22固态发酵条件进行优化,选用单因素实验确定最佳基质配方及玉米粉含量、含水量、接种量、发酵温度、发酵时间的范围。采用Box-Behnken实验设计和响应面分析方法,对球孢白僵菌BbⅢ22固态发酵条件进行优化,得出最佳固态发酵条件为:玉米粉8.22 g/L,水分38%,接种量10.5%,发酵温度25.7℃,在此条件下球孢白僵菌BbⅢ22的产孢量达到了10.578×109个/g,为优化前的3倍。The fermentation conditions for Beauveria bassiana BbⅢ22 were optimized.At first,the optimal matrix formula,corn flour concentration,water contentration,inoculation,fermentation temperature and fermentation time were determined by single factor experiments.By using Box-Behnken experimental design and response surface analytical methodology the optimum fermentation conditions were obtained： Corn flour 8.22 g/L,water content 38%,inoculation 10.5%,temperature 25.7 ℃.Under these conditions,the sporulation of BbⅢ22 was 10.578 ×109 in per garm,being increased by 2 times compared with that before optimization.

关 键 词：固态发酵 球孢白僵菌 响应面分析 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]