成年态南丰蜜橘离体再生芽培养及芽维持的蛋白谱特征  

The Study on Mature Citrus reticulita Blanco Regeneration Buds Culture in Vitro and Protein Spectral Characteristics of Buds Maintained

作　　者：彭先全[1] 涂艺声[1] 丁明华[1] 李跃进 张志强 

机构地区：[1]江西师范大学生命科学学院,江西南昌330022 [2]南丰县蜜橘良种繁育公司,江西南丰344511

出　　处：《江西师范大学学报（自然科学版）》2011年第6期579-582,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(30660020);江西省科技支撑计划[赣财教2007(173)]资助项目

摘　　要：以成年态南丰蜜橘茎段为材料,在不同激素配比的MS培养基中进行再生芽的诱导和继代增殖培养,确定了最佳的诱导培养基改良MS+6-BA0.5 mg/L+KT0.5 mg/L+NAA0.1 mg/L,把诱导培养基中的蔗糖浓度由3%提高到6%可以很好地解决再生芽的叶片发黄、脱落问题;再生芽增殖的最佳培养基为改良MS+6-BA0.4 mg/L+ZT0.1 mg/L+NAA0.05 mg/L,增殖系数达5.40.SDS-PAGE电泳结果表明33.4 kD相对分子质量的蛋白谱带出现于再生芽正常分化生长时期,可能是维持再生芽生长发育的特异蛋白组分.In this paper, the mature stem segments of Citrus reticulita Blanco were used as explants for buds induction and subculture culture on medium with different hormone combinations .The results showed that the best induction medium is improvement MS＋6-BA0.5 mg/L ＋KT0.5 mg/L ＋ NAA0.1 mg/L. The induction medium of sucrose concentration from 3% to 6% can solve the leaves of buds yellow, shedding problems; subculture proliferation medium of buds is improvement MS＋6-BA0.4 mg/L＋ZT0.1 mg/L＋NAA0.05 mg/L. Proliferation of multiple was 5.40. The results of SDS-PAGE show that 33.4kD protein is only expression in the period of the buds begin to normal growth. It probably the specific protein of buds maintained for normal growth.

关 键 词：成年茎段 南丰蜜橘 再生芽 离体培养 SDS—PAGE 

分 类 号：Q944[生物学—植物学]