大鼠心脏硫酸腺嘌呤预处理心肌酶活性变化  

CHANGE IN ENZYME ACTIVITY OF MYOCARDIUM IN RATS BY SULPHURIC ACID ADENINE PRECONDITIONING

作　　者：袁玉林[1] 郑汉巧[2] 张友云[1] 孟晓萍[1] 冯晓祥[1] 雷岳山[1] 胡成俊[1] 

机构地区：[1]湖北医科大学人体解剖学教研室,武汉430071 [2]湖北医科大学法医学教研室,武汉430071

出　　处：《解剖学杂志》2000年第1期33-36,共4页Chinese Journal of Anatomy

摘　　要：目的：实验观察经典缺血处理及硫酸腺嘌呤处理大鼠心肌细胞色素氧化酶和过氧化氢酶的活性变化。方法：采用ＳＤ雄性大鼠２４只，分４组：假手术组、缺血再灌注组、经典缺血预处理组、硫酸腺嘌呤预处理组。术后速取左心室前壁肌行冰冻切片，以 ＤＡＢ法测定细胞色素氧化醇和过氧化氢酶的活性。依Ｒｉｄｉｔ法行显著性检验。结果：缺血再灌注组大鼠心肌过氧化氢酶和细胞色素氧化酶损伤性反应明显，经典缺血预处理组和硫酸腺嘌呤预处理组细胞色素氧化酶、过氧化氢酶损伤性反应较之明显减轻（Ｐ＜０．０５）。结论：经典缺血预处理和硫酸腺嘌呤预处理对缺血再灌注大鼠心肌损伤有明显保护作用。To observe the change in cytochrome oxidase(CCO) and catalase (CAT) activities in rat myocardium by ischemic preconditioning and sulphuric acid adenine preconditioning. Methods: Twenty-four male rats were divided into 4 groups: sham-operation, ischemic reperfusion, ischemic preconditioning and sulphuric acid adenine preconditioning. Left ventricular myocardium frozen was sectioned and CCO, CAT activities were tested by DAB method. The significane was expressed by Ridit method. The results showed that CCO and CAT activities in ischemic reperfusion group were sigificantly lower than it suplphuric acid adenine preconditioning group and ischemic preconditioning proup(p<0. 05). Conclusions: Sulphuric acid adenine and ischemic preconditioning can protect myocardium from ischemic reperfusion in rats.

关 键 词：心肌缺血 预处理 硫酸腺嘌呤 心肌酶活性 

分 类 号：R363[医药卫生—病理学]