钙网蛋白的ELISA检测方法的建立及其在膀胱癌诊断中的应用研究  

Establishment of ELISA method for calreticulin and its application in the diagnosis of bladder urothelial carcinoma

作　　者：许瑞环[1] 陈章权[2] 罗艺[1] 曾建蓉[1] 胡劲辉[1] 

机构地区：[1]深圳市龙岗中心医院中心实验室,广东518116 [2]广东医学院临床免疫学教研室,广东东莞523808

出　　处：《国际检验医学杂志》2011年第20期2323-2324,2327,共3页International Journal of Laboratory Medicine

基　　金：广东省医学科研基金资助项目(A2008653);广东省湛江市科技招标项目

摘　　要：目的建立钙网蛋白的ELISA检测方法,同时探讨其在膀胱癌诊断中的应用。方法应用制备的钙网蛋白的多克隆抗体包被酶标板,同时制备其与HRP交联物,应用双抗体夹心法构建钙网蛋白的ELISA检测方法。应用构建的方法检测膀胱癌患者尿液钙网蛋白的浓度,同时采用发光法检测尿液中CA199和CYFRA21-1的浓度。结果 ELISA法检测膀胱癌尿液钙网蛋白阳性率为100.00%,良性病变组阳性率为31.25%,对照组其阳性率为0.00%。尿液钙网蛋白,其测定特异性为83.87%,敏感性为100.00%;尿液CYFRA21-1,当临界值为4.15ng/mL时,其诊断膀胱癌的特异度为77.55%,灵敏度为43.48%。结论应用建立的ELISA方法检测尿液钙网蛋白,可用于膀胱癌的诊断、监测及预后评估。Objective To establish an ELISA method of calreticulin and explore its application in the diagnosis of bladder urothelial carcinoma.Methods Coat plate with prepared polycolonal antibody for calreticulin was used,meanwhile HRP labeled polycolonal antibdy was prepared.Established a sanwich antibody ELISA method which was used to test the concentration of calreticulin.The concentration of CYFRA21-1 and CA19-9 were tested by chemiluminescence method.Results The positive rate of calreticulin for bladder urothelial carcinoma in urine was 100.00% with ELISA,31.25% for urological patients without urothelial carcinoma and 0.00% for health people.The specificity of calreticulin for bladder urothelial carcinoma was 83.87%,and its sensitivity was 100.00%.But for CYFRA21-1,these were 77.55% and 43.48% respectively when the limit was 4.15 ng/mL.Conclusion Calreticulin in the urine can be tested by established method of ELSA,which can be useful for diagnosis,monitoring and prognosis for bladder urothelial carcinoma.

关 键 词：膀胱肿瘤 钙网蛋白 酶联免疫吸附测定 尿 

分 类 号：R737.14[医药卫生—肿瘤]