LyGD1基因转染对肺腺癌A549细胞生长及放化疗敏感性的影响  

Gene Transfection of LyGDI into A549 Cell Line and Its Effect on Cell Growth and Sensitivity to Chemotherapy and Radiotherapy

作　　者：曹丽丽[1] 段广新[1] 董玉金[1] 王兴丹[1] 周新文[1] 

机构地区：[1]苏州大学医学部放射医学与防护学院放射毒理学教研室江苏省放射医学与防护重点实验室,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2011年第6期870-873,878,共5页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30570548;81071878)

摘　　要：目的研究转染LyGDI基因对肺腺癌A549细胞生长及放、化疗敏感性的影响。方法用脂质体介导的方法,将构建于pEGFP载体上的LyGDI及空载体pEGFP-C1转入A549细胞,用G418筛选出稳定表达株,RT-PCR法了解LyGDI基因在转染细胞中的表达情况;用平板克隆形成实验研究细胞生长情况;体外划痕试验观察对细胞侵袭及转移能力的影响;甲基偶氮唑蓝法、克隆形成实验分别研究细胞对放、化疗的敏感性。结果转染细胞中有相应融合基因表达;与转染前比较,基因转染后,阳性细胞尤其是表达LyGDI的A549细胞克隆形成能力显著降低(P<0.01),对常用化疗药物顺铂和放射线敏感度显著提高(P<0.05或<0.01)。结论建立了稳定转染细胞株;LyGDI转入抑制了A549细胞的生长能力,且增加了细胞对放、化疗的敏感性。Objective To express LyGDI in A549 cells by using gene transfection,and to observe its effect on cell growth and cell sensitivity to chemotherapy drugs and radiation.Methods The LyGDI genes,which were constructed with the pEGFP vector,and the empty vector pEGFP-Cl were transfected into A549 cells respectively.The stable expression cells were screened by G418.LyGDI expression levels in the transfected A549 cells were assessed at mRNA level by RT-PCR.The clone-forming assay was done to detect the effect of LyGDI on the growth of A549 cells.The in vitro scratch assay was done to observe the effects on the invasion and metastasis of A549 cells.The MTT assay and clone-forming assay were performed to test effects of LyGDI on sensitivity of the A549 cells to chemotherapy drugs and radiation respectively. Results After transfection,positive cell,especially A549 cell expressing LyGDI showed an decrease in colony-forming ability and higher sensitivity to DDP and radiation（all P〈0.01）.Conclusion The stable expression cells are established;the introduction of LyGDI inhibits growth,ability of invasion and metastasis,and enhance chemotherapy drugs-sensitivity and radiation-sensitivity of A549 cells.

关 键 词：LYGDI A549细胞 稳定转染 细胞生长 化疗敏感性 放疗敏感性 

分 类 号：R734.2[医药卫生—肿瘤]