^(131)I-Herceptin在乳腺癌裸鼠模型中的体内分布  

Biodistribution of ^(131)I-Herceptin in Breast Cancer Xenograft

作　　者：杨志学[1] 蒋国勤[1] 邢春根[1] 危少华[1] 刘增礼[2] 

机构地区：[1]苏州大学附属第二医院普外科,江苏苏州215004 [2]苏州大学附属第二医院核医学科,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2011年第6期909-912,910+909,共4页Suzhou University Journal of Medical Science

摘　　要：目的建立Her-2高表达乳腺癌动物模型,了解小鼠体内^(131)I-Herceptin的生物分布。方法以对数生长期的SK-BR-3细胞皮下接种BALB/c-neu裸鼠建立动物模型。测量小鼠注射^(131)I-Herceptin后4、12、24、48 h每克各组织每分钟的放射性计数(cpm/g),并计算肿瘤与非肿瘤组织放射性计数比值(T/NT)及每克组织放射性计数占注射剂量放射性计数的百分比(%ID/g)。结果 SK-BR-3细胞皮下接种BALB/c-neu裸鼠后成瘤率96%。实验组与对照组T/NT值及%ID/g比较,差异有统计学意义(P<0.05或<0.01)。结论以SK-BR-3细胞皮下接种裸鼠成瘤率高,^(131)I-Herceptin在肿瘤组织中浓聚明显。Objective To establish Her-2 positive SK-BR-3 human breast cance xenografts in athymic mice. To explore the biologic distribution of 131I-Herceptin in human breast cancer xenografts. Method Implant SK-BR-3 cells subcutaneously to BALB/c-neu athymic mice to establish animal model. To measure the radiocounting per minute（epm） of every organ on a arithmometer at 4,12,24,48 h postinjection of 131I-Herceptin or 131I-mIgG , then to gain the T/NT ratios and the uptakes percentage per gram of the injection dose （% ID/g）. Results After subcutaneously planted, a 96% of tumor formming rate was achieved . Compared with the control group, bigger T/NT and % ID/g was obtained in the experimental group （P 〈 0.05 and 〈 0.01 ）. Conclusion A high tumor formming rate can be get by implanting SK-BR-3 cells subcutaneously to athymic mouse, 131I-Hereeptin is obviously concentrated in tumor tissues.

关 键 词：乳腺癌 ~131I-Herceptin 生物学分布 裸鼠 

分 类 号：R737.9[医药卫生—肿瘤]