紫苏子叶组织培养植株再生的研究  

Study on the Plant Regeneration in Tissue Culture of Perilla frutescens Cotyledon

作　　者：李会珍[1] 刘培培[1] 张志军[1] 刘西亮[1] 

机构地区：[1]中北大学化工与环境学院生命科学系,山西太原030051

出　　处：《安徽农业科学》2011年第22期13369-13371,共3页Journal of Anhui Agricultural Sciences

基　　金：山西省回国留学人员科研资助项目(2009065)

摘　　要：[目的]以紫苏无菌苗子叶为外植体研究紫苏芽再生频率。[方法]在MS培养基中添加不同浓度配比的6-BA和IAA,给予不同黑暗培养时间2、4,-D浓度和预培养时间,研究各因素对紫苏芽苗分化的影响。[结果]子叶在分化培养基MS+1.0 mg/L 6-BA+1.5mg/L IAA中培养可获得高达80.01%的再生频率0,.10 mg/L 24,-D的分化培养基预培养3 d,黑暗培养12 d后再转入光照培养效果最好。[结论]该试验可为紫苏的组培繁殖提供参考。[Objective] To study the regeneration of cotyledons from Perilla frutescens.[Method] By adding different concentration of 6-BA and IAA into the MS medium,the effects of different darkness preculture time,2,4-D concentrations and preculture duration on the regeneration of Perilla frutescen were inspected.[Result]As high as 80.01% regeneration frequency was achieved in the medium of MS＋1.0 mg/L 6-BA＋1.5 mg/L IAA,and the best culture effect was realized under the 3 d preculture of cotyledon in the medium of 0.1 mg/L 2,4-D,followed by 12 d darkness culture before being transferred to light conditions.[Conclusion] The experiment provided references for the tissue culture of Perilla frutescens.

关 键 词：紫苏 子叶 再生 

分 类 号：S565.8[农业科学—作物学]