Bax基因对人胃癌耐药细胞多药耐药性的逆转作用  

Reversal of multidrug resistance of gastric cancer cell line SGC7901/VCR by Bax gene transduction

作　　者：赵燕秋[1] 肖冰[2] 樊代明[1] 

机构地区：[1]第四军医大学西京医院消化科,西安710032 [2]第一军医大学南方医院消化科

出　　处：《中华消化杂志》2000年第1期8-10,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金杰出青年基金!资助项目 (3952 50 2 0 )

摘　　要：目的　研究凋亡相关基因Bax对胃癌多药耐药细胞的多药耐药性的逆转作用。方法　构建含有人BaxcDNA全长的真核表达载体 pBK Bax ,经脂质体导入缺乏Bax蛋白表达的人胃癌多药耐药细胞系SGC790 1/VCR中。Western印迹观察Bax基因在转导细胞中的表达 ,MTT法检测Bax转导细胞与空载体转导细胞对化疗药物的敏感性。结果　成功构建了Bax的真核表达载体 ,Bax转导细胞能稳定表达Bax蛋白 ,与空载体转导细胞相比 ,Bax转导细胞对长春新碱 (vincristine ,VCR)、阿霉素 (adri amycin ,ADR)、丝裂霉素 (mitomycin ,MMC)的敏感性显著增加 (P <0 .0 1)。 结论　Bax基因转导对胃癌多药耐药细胞SGC790 1/VCR的多药耐药性具有逆转作用。Objective To investigate the effect of Bax gene transduction on the multidrug resistance of gastric cancer cell line SGC7901/VCR. Methods Construct a eukaryotic vector carrying the full length of Bax cDNA, then transfect it into SGC7901/VCR cells lacking Bax protein expression by lipofectamine. After G418 selection, Western blot was used to detect the Bax protein expression in Bax transducted cells; the drug sensitivity between Bax transfectants and vector transducted cells were compared with MTT assay only. Results The authors successfully constructed Bax eukaryotic expression vector. Bax protein was stably expressed in Bax transfectants. MTT results showed Bax transfectants were more sensitive to VCR, ADR and MMC than vector transducted cells singly. Conclusion Bax gene transduction reversed the multidrug resistance of gastric cancer cell line SGC7901/VCR to some extent.

关 键 词：BAX基因 转导 胃癌 多药耐药 药物疗法 基因治疗 

分 类 号：R735.2[医药卫生—肿瘤]