VP-16诱导PC-3细胞凋亡和p53基因表达的研究  

VP-16 induced programmed cell death and expression of p53 gene in PC 3 cells

作　　者：李登欣[1] 张建业[1] 张莲英[1] 何维敬[1] 

机构地区：[1]山东医科大学生化教研室,济南250012

出　　处：《中华泌尿外科杂志》2000年第1期34-36,共3页Chinese Journal of Urology

基　　金：山东省科委基金!资助(960079)

摘　　要：目的　研究ＶＰ１６ 诱导雄激素非依赖型前列腺癌细胞ＰＣ３ 凋亡和抑癌基因ｐ５３ 表达的关系。　方法　以末端标记法（ＴＵＮＥＬ） 和流式细胞术（ＦＣＭ） 研究ＰＣ３ 细胞凋亡及细胞周期分布；以免疫组化方法研究ｐ５３ 蛋白的表达。　结果　末端标记法显示凋亡的ＰＣ３ 细胞呈明显的形态学改变，流式细胞术和免疫组化检测结果表明，随ＶＰ１６ 作用浓度的增加和作用时间的延长，ＰＣ３ 细胞的凋亡率和其ｐ５３ 基因表达阳性的细胞百分率逐渐增高。　结论　ＶＰ１６ 能够诱导ＰＣ３ 细胞凋亡和增加ｐ５３ 基因表达，且具有作用浓度和作用时间依赖性。Objective To study the relationship between apoptosis induced by etoposide in androgen independent PC 3 cells and to evaluate the expression of p53 tumor suppression gene. Methods TUNEL(TdT mediated x dUTP nick end labeling) and FCM (flow cytometry) were applied to investigate the apoptosis in PC 3 cells and distribution of cell cycle.p53 protein was detected immunohistochemically. Results Apparent apoptic morphological changes in PC 3 cells were observed by TUNEL.FCM and immunohistochemical assay indicated that the percentage of apoptic cells and the positive cells of p53 expression were increased with the increasing of exposure time and concentration of VP 16. Conclusions VP 16 can induce the programmed cell death of PC 3 cells and enhances the expression of p53 gene.

关 键 词：前列腺肿瘤 癌 细胞凋亡 P53 基因表达 

分 类 号：R737.25[医药卫生—肿瘤]