鼻咽癌中浸润的淋巴类细胞与瘤细胞凋亡的关系(英文)  

Correlation Between Infiltrating Lymphoid Cells and Neoplastic Cell Apoptosis in Nasopharyngeal Carcinoma

作　　者：宗永生[1] 李智[1] 刘克拉[1] 买世娟[1] 梁英杰[1] 

机构地区：[1]中山医科大学病理学教研室,广东广州510089

出　　处：《中山医科大学学报》2000年第1期6-7,共2页Academic Journal of Sun Yat-sen University of Medical Sciences

摘　　要：:【目的】研究鼻咽癌中浸润的淋巴类细胞与癌细胞凋亡间的相关性。【方法】收集中山医科大学病理学教研室38例未经治疗的鼻咽癌活检标本。应用TUNEL(TdTmediateddUTPNickEndLabeling)方法定量检测凋亡癌细胞计数(每5000个鼻咽癌细胞中的凋亡细胞数)。以免疫组化法分别检测T细胞,单核/巨噬细胞,B细胞和树突状细胞。【结果】①在未经治疗的鼻咽癌活检标本各例间癌细胞凋亡计数差异很大,平均为(129±139)/5000;②凋亡癌细胞计数与T细胞指数(I,即平均每个高倍视野浸润的T淋巴细胞数)和单核/巨噬细胞指数(moncytemacrophageindex,MMI,即平均每个高倍视野浸润的单核/巨噬细胞数)均呈正相关,P<0.05,但凋亡癌细胞计数与B细胞指数(平均每个高倍视野浸润的B淋巴细胞数)和树突状细胞指数(平均每个高倍视野浸润的树突状细胞数)无相关性,P>0.05。【结论】①代表细胞介导免疫的浸润性T淋巴细胞可以诱导鼻咽癌细胞凋亡;②浸润的单核/巨噬细胞可能主要是癌细胞凋亡的诱导者也可能部分是凋亡的后果;Objective To investigate the correlation between infiltrating lymphoid cells and neoplastic cell apoptosis in nasopharyngeal carcinoma (NPC). Methods 38 untreated NPC biopsies were collected from the Department of Pathology, Sun Yat sen University of Medical Sciences. TUNEL (TdT mediated dUTP Nick End Labeling) method was performed for quantitating the apoptosis count (AC, numbers of apoptotic cell from among 5 000 NPC cells). DAKO LSAB kit (K 0681) and respective primary antibodies were used for detecting reactive T lymphocyte, monocyte/macrophage, pan B lymphocyte, and accessory dendritic cell immunohistochemically. The amount of infiltrating lymphoid cells was indicated in terms of TCI (T cell index), MMI (monocyte/macrophage index), BCI (B cell index), and DCI (dendritic cell index) by their cell numbers per one average high power field, respectively. Results ①The AC varied greatly from case to case in untreated NPCs, the mean value being (12.9±13.9)/5 000; ②The AC was both positively correlated with TCI (P<0.05) and MMI (P<0.05), but not with BCI (P>0.05) and DCI (P>0.05). Conclusions ①The infiltrating T lymphocytes representing cell mediated immunity are able to induce NPC cell apoptosis; ②The infiltrating monocytes/macrophages might principally be an apoptosis inducer or as a consequence of degraded apoptotic debris; ③The infiltrating B lymphocytes representing humoural immunity and antigen presenting dendritic cells as well are not directly correlated with NPC cell apoptosis.

关 键 词：鼻咽癌 淋巴类细胞 瘤细胞 细胞凋亡 病理学 

分 类 号：R739.63[医药卫生—肿瘤]