三七皂苷Rg1抑制脂多糖诱导的小胶质细胞激活  

Ginsenoside Rg1 inhibiting the lipopolysaccharide-induced activation of microglial cells

作　　者：宗一[1] 戴纪男[1] 孙俊[1] 杨萍[1] 张伟[2] 艾青龙[2] 陆地[1] 

机构地区：[1]昆明医学院人体解剖学教研室,昆明650500 [2]昆明医学院第一附属医院神经内科,昆明650011

出　　处：《解剖学报》2012年第1期13-18,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30860336;30560170);云南省科技厅-昆明医学院联合资助项目(2008CD016;2010CD156;2011FB177);云南省中青年学术和技术带头人后备人才培养资助项目(2009CI033);云南省应用基础研究重点资助项目(2008CC007);昆明医学院研究生创新基金资助项目(2011J01)

摘　　要：目的探讨三七皂苷Rg1(Rg1)对脂多糖(LPS)诱导的小胶质细胞系BV-2细胞炎性因子释放的抑制作用。方法用LPS刺激BV-2细胞构建炎症模型,采用四甲基偶氮唑盐比色法检测Rg1对BV-2细胞活力的影响,免疫荧光染色和反转录PCR方法检测不同浓度Rg1(10、20、40μmol/L)对细胞炎性蛋白酶诱导型一氧化氮合酶(iNOS)和环氧合酶-2(COX-2)、细胞炎性因子肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)、炎性信号分子NF-κB蛋白与mRNA的表达变化。结果不同浓度的Rg1在转录水平和翻译水平上明显抑制了LPS诱导的细胞炎性蛋白酶iNOS和COX-2、细胞炎性因子TNF-α和IL-1β与炎性信号分子NF-κB的上调,并且iNOS、COX-2和NF-κB的表达呈剂量依赖性。结论 Rg1可通过调控LPS诱导的小胶质细胞系BV-2细胞炎性因子释放从而抑制小胶质细胞激活,发挥抗神经炎症的作用。Objective To investigate whether ginsenoside Rgl （ Rgl ） inhibits the expression of potentially pro- inflammatory cytokines by cultured murine microglial BV-2 cells stimulated with lipopolysaccharide （LPS）. Methods An inflammatory cell model was structured by LPS-stimulated microglial BV-2 cells. The inflammatory ceils were treated with Rgl （10,20 and 40μmol/L） prior to LPS exposure. The effects on the mRNA and protein levels of pro-inflammatory enzymes, inducible nitric oxide synthase （iNOS）, cyclooxygenase-2 （COX-2）, pro-inflammatory cytokines, tumor necrosis factor-α （TNF-α） , interleukin-1β （ IL-1β） , and inflammatory signaling proteins nuclear factor-κB （NF-κB） were analysed by reverse transcription-polymerase chain reaction （RT-PCR） and immunofluorescence staining assay. The effects of Rgl on viability of BY-2 cells were measured by MTT assay. Results The results indicated that LPS-induced iNOS and COX-2 protein and mRNA expression levels decreased significantly by Rgl in a concentration-dependent manner. Rgl also had an effect on the expression of TNF-α, IL-1β and NF-κB through transcriptional and translational inhibition. In addition, Rgl dose-dependently decreased the increasing expression of iNOS, COX-2 and NF-KB protein and mRNA stimulated by LPS. Conclusion Rgl exerts anti-neuroinflammatory via regulating microglial BV-2 cells to release inflammatory factors.

关 键 词：三七皂苷RG1 脂多糖 炎性因子 反转录-聚合酶链式反应 免疫荧光 BV-2细胞 

分 类 号：R742.1[医药卫生—神经病学与精神病学]