卵巢切除大鼠睑板腺雌激素α、β受体表达与组织病理学改变的相关性  

Correlation of the expression of estrogen receptors α and β with histopathology changes in the meibomian gland of the ovariectomized rat

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作  者:李联祥[1] 金东岭[2] 高金生[2] 王黎光[3] 刘现军[2] 

机构地区:[1]河北工程大学医学院人体解剖学与组织胚胎学教研室基础医学实验室,河北邯郸056002 [2]河北工程大学医学院病理学教研室,河北邯郸056002 [3]河北工程大学医学院生理学教研室,河北邯郸056002

出  处:《解剖学报》2012年第1期103-108,共6页Acta Anatomica Sinica

基  金:河北省科学技术与发展计划基金资助项目(10276105D-3)

摘  要:目的探讨卵巢切除后大鼠雌激素α、β受体(ERα、ERβ)表达与睑板腺组织病理学改变的相关性。方法取48只成年SD雌性大鼠,随机分为正常对照组、假手术组和去势组(去卵巢组),每组16只,12周后处死大鼠,免疫组织化学检测睑板腺组织ERα和ERβ表达变化,光学显微镜和透射电子显微镜观察睑板腺组织学改变,应用放射免疫方法检测血清雌二醇水平。结果去势组ERα表达与正常对照组间差异无统计学意义(P>0.05),但ERβ表达弱于正常对照组(P<0.01)。正常对照组ERα/ERβ表达比值为1.06,而去势组ERα/ERβ表达比值为1.83。光镜下观察去势组睑板腺腺泡组织结构排列紊乱,细胞体积变小,腺泡呈萎缩状态。电镜下观察去势组睑板腺腺泡上皮的基底细胞体积变小,细胞核形态变异多样,呈不规则形、肾形或长杆状;过渡细胞的体积变小,脂滴减少,线粒体肿胀,线粒体嵴结构破坏,呈空泡化或云雾状改变,并可见大量嗜锇物质形成的圆形或不规则形电子致密的板层小体或髓鞘样结构。结论卵巢切除后睑板腺组织病理学改变可能与ERβ表达减弱或ERα/ERβ比值失调有关。Objective To study the correlation of the estrogen receptors α and β expression level with histopathology change in the meibomian gland of the ovariectomized rats. Methods Forty-eight adult female SD rats were randomly divided into three groups: the normal control group (16 rats), the sham operated group( 16 rats) and the ovariectomized group (16 rats). After 12 weeks, all rats were killed and the tarsus tissues were excised. Expression of ERα and ERβ of meibomian glands were studied with immunohistochemistry. Histology of the glands was observed under a light microscope and a transmission electron microscope. The estrogen level of blood serum was examined with radioimmunoassay. Results There was no significant difference of the ERα expression level between the ovariectomized group and the control group ( P 〉 0.05 ). The ERβ expression level in the ovariectomized group was weaker than that of the control group (P 〈 0. 01 ). ERα / ERβ expression ratio was 1.06 in the control group and 1.83 in the ovariectomized group. The light microscope examination showed that the tissue and structure of meibomian gland acinar were abnormal in shape, cell size was smaller, and acinars were atrophy in the ovariectomized group. Under the transmission electon microscope, the gland acinar epithelia ceils of the basal meibomian gland were small size, and the nuclei appeared irregular or kidney-like in shape. Transitional cells became small, the fatty drop reduced, and the mitochondrial swelling and mitochondrial cristae structural damage e. g. , a vacuole or clouds changes, and a lot of medullary-like structure in the cytoplasm were existed in the ovariectomized group. Conclusion After ovariectomized, the histopathology changes of meibomian gland may be involved in decrease of ERI3 expression level or the ratio change of ERα / ERβ expression.

关 键 词:睑板腺 雌激素受体亚型 干眼症 卵巢切除 组织病理学 透射电镜 大鼠 

分 类 号:R777.13[医药卫生—眼科] R335[医药卫生—临床医学]

 

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