微管蛋白酪氨酸连接酶类似物12和硝基化酪氨酸对前列腺癌细胞生长的影响  

Effect of tubulin tyrosine ligase like 12 and nitrotyrosine on growth of prostate cancer cells

作　　者：李雅冬[1] 张劲松[1] 杨凯[1] 张福军[1] 陈睿[1] 洪苏玲[2] 

机构地区：[1]重庆医科大学附属第一医院颌面外科,重庆400016 [2]重庆医科大学附属第一医院耳鼻喉头颈外科,重庆400016

出　　处：《第二军医大学学报》2012年第1期1-4,共4页Academic Journal of Second Military Medical University

基　　金：重庆市卫生局医学科研计划项目(2011-2-013)~~

摘　　要：目的研究微管蛋白酪氨酸连接酶类似物12(TTLL12)和硝基化酪氨酸对前列腺癌细胞生长的影响。方法利用正常前列腺上皮细胞株PWR1E、RWPE1和前列腺癌细胞株DU145,通过蛋白质印迹分析检测TTLL12和硝基化酪氨酸微管蛋白的表达量,用MTT实验检测细胞的增殖情况。结果在硝基化酪氨酸的作用下,DU145细胞的硝基化酪氨酸微管蛋白表达量低于PWR1E和RWPE1细胞(P<0.05)。和对照组(以含800μmol/L盐酸培养)相比,实验组(以含800μmol/L硝基化酪氨酸的培养液培养)PWR1E和RWPE1细胞增殖受到抑制(P<0.05),而DU145细胞无明显改变(P>0.05)。在有效沉默TTLL12后,实验组(用siTTLL-12转染)的硝基化酪氨酸微管蛋白表达量高于对照组(用siControl转染,P<0.05);实验组内,硝基化酪氨酸使DU145细胞增殖受到抑制(P<0.05),而对照组内,硝基化酪氨酸对细胞增殖改变较小(P>0.05)。结论 TTLL12可使前列腺癌细胞逃避硝基化酪氨酸的打击,从而使前列腺癌细胞逃脱机体监控作用,获得异常增殖机会。Objective To study the effect of tubulin tyrosine ligase like 12 （TTLL12） and nitrotyrosine on the growth of prostate cancer cells. Methods TTLL12 and α-tubulin nitrotyrosination （N-tubulin） were detected by Western blotting analysis in normal prostate epithelial cell lines PWR1E and RWPE1, and prostate cancer cell line DU145; the proliferation of cells was observed by MTT assay. Results The expression of N-tubulin was significantly lower in DU145 cells than those in PWR1E and RWPE1 cells after treatment with nitrotyrosine （P〈0. 05）. The proliferation of PWRIE and RWPE1 cells treated with nitrotyrosine （cultured with 800μmol/L nitrotyrosine） were significantly inhibited compared with the control （cultured with 800 μmol/L hydrochloric acid, P〈0.05）, while there was no visible change in the proliferation of DU145 cells. N-tubulin level was significantly higher in the experimental group （transfected with siTTLL-12） than the control group （transfected with siControl） after effective silence of TTLL12 （P〈0. 05）; the proliferation of DU145 cells treated with nitrotyrosine in the experimental group was significantly inhibited （P〈0.05）, while there was no visible change in cell proliferation in the control group （P〉0.05）. Conclusion TTLL12 allows an escape of prostate cancer cells from the treatment of nitrotyrosine,leading to the escape of prostate cancer cells from the body＇s surveillance system and acquirement of their abnormal proliferation.

关 键 词：微管蛋白酪氨酸连接酶类似物12 硝基化酪氨酸 前列腺肿瘤 

分 类 号：R737.25[医药卫生—肿瘤]