肝癌患者血清高迁移率族蛋白B1的表达及与p53和临床病理的相关性  

Serum HMGB-1 level and its correlation with p53 and clinical pathology in patients with liver cancer

作　　者：陈科济[1] 范飞[1] 朱斌[1] 马优钢[1] 张永杰[1] 

机构地区：[1]第二军医大学东方肝胆外科医院胆道二科,上海200438

出　　处：《第二军医大学学报》2012年第1期103-105,共3页Academic Journal of Second Military Medical University

摘　　要：目的探讨肝癌血清高迁移率族蛋白B1(HMGB-1)的表达及与p53的相关性和临床病理特征的关系。方法酶联免疫吸附剂测定法(ELISA)检测20例肝细胞癌、20例肝内胆管细胞癌患者和20例健康志愿者血清HMGB-1和p53表达。结果血清HMGB-1在肝细胞癌组[(83.8±17.4)ng/ml]和胆管细胞癌组[(95.9±21.4)ng/ml]的表达均高于健康对照组[(8.4±2.6)ng/ml],差异均有统计学意义(P<0.05);血清p53在肝细胞癌组[(251.0±45.6)pg/ml]和胆管细胞癌组[(267.8±47.4)pg/ml]的表达均高于健康对照组[(160.0±28.4)pg/ml],差异均有统计学意义(P<0.05)。HMGB-1和p53的表达在肝细胞癌组和肝内胆管细胞癌组血清中均呈正相关(r=0.660 3,P<0.05;r=0.614 2,P<0.05);血清HMGB-1和p53水平与患者年龄、性别、肿瘤大小和肿瘤数目无相关性(P>0.05),与肿瘤TNM分期、分化程度、淋巴转移情况相关(P<0.05)。结论肝癌血清HMGB-1的表达与p53具有相关性,二者共同影响肝癌临床病理。Objective To investigate the serum level of high mobility group box-1（HMGB-1） in patients with liver cancer and its correlation with p53 and clinical pathological features of liver cancer. Methods Serum levels of HMGB-1 and p53 were determined by ELISA in 20 patients with hepatocellular carcinoma, 20 with cholangiocellular carcinoma and 20 healthy volunteers. Results Serum HMGB-1 levels in hepatocellular carcinoma patients （[83.8± 17.4] ng/ml） and cholangiocellular carcinoma patients （[95.9±21.4] ng/ml） were significantly higher than that in the healthy volunteers （[8.4± 2.6] ng/ml, P〈0.05）. Serum p53 levels in hepatocellular carcinoma patients （[251. 0± 45. 6] pg/ml） and cholangiocellular carcinoma patients （[267.8±47.4] pg/ml） were also significantly higher than that in the healthy volunteers （[160.0±28.4] pg/ml, P〈0.05）. Serum HMGB-1 level was positively correlated with the serum p53 level in the hepatocellular carcinoma patients （r=0. 660 3, P〈0.05） and cholangiocellular carcinoma patients （r=0. 614 2, P〈0.05）. Serum HMGB-1 and p53 levels were not correlated with the age, gender, tumor size, or number of tumors （P〉0.05）, but were correlated with the TNM stage, differentiation degree and metastasis status （P〈0.05）. Conclusion Serum HMGB-1 level is positively correlated with p53, and both HMGB-1 and P53 influence the pathogenesis of liver cancer.

关 键 词：肝肿瘤 HMGB-1蛋白质 肿瘤抑制蛋白质P53 临床病理学 酶联免疫吸附测定 

分 类 号：R735.7[医药卫生—肿瘤]