9-顺式维甲酸对甲状腺鳞癌SW579细胞周期及CYCLIND1、CDK4表达的影响  

Effects of 9-cis-retinoic acid on cell cycle and expressions of CYCLIND1and CDK4 in thyroid squamous cell carcinoma cell line SW579

作　　者：张秀梅[1] 刘超[1] 闫纪亮[1] 赵颂[1] 王翠瑶[1] 肖建英[1] 

机构地区：[1]辽宁医学院生物化学教研室,辽宁锦州121000

出　　处：《基础医学与临床》2012年第2期186-190,共5页Basic and Clinical Medicine

基　　金：辽宁省教育厅重点实验室项目(LS2010101)

摘　　要：目的探讨9-顺式维甲酸(9-cisRA)对甲状腺鳞癌细胞系SW579细胞周期及周期因子表达的影响。方法分别以不同浓度的9-cisRA处理SW579细胞系,采用MTT比色法测定细胞增殖活性;用流式细胞仪检测细胞周期;用RT-PCR方法检测SW579细胞CDK4、CYCLIND1 mRNA的表达水平;用Western blot法检测SW579细胞CDK4、CYCLIND1的蛋白表达。结果 9-cisRA作用后,各组细胞均出现显著的生长抑制作用,生长抑制率明显升高,并呈剂量依赖性。流式细胞仪分析,各组细胞均随着药物浓度的升高,S期细胞在细胞周期中所占比例逐渐减少,G1期细胞则明显增加(P<0.01),细胞滞留在G1期。经不同浓度9-cisRA处理的细胞CDK4 mRNA和蛋白表达水平没有明显变化;CYCLIND1的mRNA和蛋白表达水平明显下调(P<0.01)。结论 9-cisRA显著抑制SW579细胞生长,可能与抑制CYCLIND1的表达并将细胞阻滞于G1期有关。Objective To study the effects of 9-cis retinoic acid （9-cisRA） on cell cycle and expressions of CYCLIND1 and CDK4 in thyroid squamous cell carcinoma cells （SW579）. Methods Cells were treated with differ- ent doses of 9-cisRA, the proliferation of the cells was observed with MTT and the cell cycle was measured by flow cytometry ;the CDK4 and CYCLIND1 mRNA expression levels were determined by RT-PCR;CDK4 and CYCLIND1 protein expression were determined by Western blot. Results After cells were treated with 9-cisRA the prolifera- tion index decreased markedly with a dose-dependent manner. Flow cytometry analysis showed the percent of G1 phase cells was significantly higher than that of the control groups and the percent of S phase cells was lower than that of the control groups. The cell cycle was arrested at G1 stage;The mRNA and protein expression of the CDK4 was not changed ; the mRNA and protein expression of CYCLIND1 was descreased dramatically （ P 〈 0. 01 ）. Con- clusion 9-cisRA inhibits the proliferation of SW579 cell in a dose-dependent manner, It is related with down-reg-ulation of CYCLIND1 and arresting SW579 cell at G1 phase.

关 键 词：9-顺式维甲酸 SW579 细胞周期 CDK4 CYCLIND1 

分 类 号：R736.1[医药卫生—肿瘤]