不同类型菜地土壤中3种磺胺类抗生素污染特征研究  

Study on Pollution Characteristic of Three Sulfonamides Antibiotics in Different Soils of Vegetable Plot

作　　者：陈海燕[1] 花日茂[1] 李学德[1] 胡忠立[1] 

机构地区：[1]安徽农业大学资源与环境学院,安徽合肥230036

出　　处：《安徽农业科学》2011年第23期14224-14226,14229,共4页Journal of Anhui Agricultural Sciences

基　　金：安徽省科技厅重点实验室项目

摘　　要：[目的]利用振荡、超声提取-液液萃取-高效液相色谱荧光检测器检测合肥市不同类型菜地土壤中3种磺胺类抗生素的含量,为土壤中磺胺类药物的检测提供依据。[方法]土样采用0.1 mol/L NaOH和甲醇(5∶5)混合溶液提取,二氯甲烷液相萃取,采用高效液相色谱荧光检测器,以乙腈和含0.5%乙酸水溶液作为流动相,激发波长(Ex)为405 nm,发射波长(Em)为495 nm,对样品进行检测。[结果]3种磺胺类抗生素在土壤中的检出限为2.5μg/kg;土壤样品中3种磺胺类抗生素添加浓度在2.5～50.0μg/kg,平均回收率为74.17%～104.35%,RSD均小于10%。所有土样中单种磺胺类抗生素检出率为16.67%～44.44%,平均含量为3.43～3.89μg/kg。[结论]4类菜地土壤中3种SAs的含量顺序为:养鸡场附近菜地>普通农家菜地>有机蔬菜基地>绿色蔬菜基地,表明土壤中磺胺类抗生素污染主要源自粪肥的施用。[Objective]The study aimed to detect three sulfonamides in different soils of vegetable plot in Hefei City by the oscillations,ultrasonic extraction-liquid extraction-HPLC fluorescence detector so as to provide the basis for the detection of sulfonamides in the soil.[Method]The soil samples were firstly extracted by a 5∶ 5（V∶ V） mixture solution of methanol and 0.1 mol/L NaOH,then extracted by dichloromethane and finally the samples were detected by using HPLC fluorescence detector,with the acetonitrile and 0.5% acetic acid as the mobile phase,and Ex=405 nm,Em=495 nm as the detective wavelength.[Result]The detection limit of 3 sulfonamides antibiotics in soils were 2.5 μg/kg.The average recoveries were 74.17%-104.35% and RSDs were lower than 10% for all the soil samples added with 2.5-50.0 μg/kg of sulfonamides.The detection limit of single sulfonamides antibiotic in all soil samples was 16.67%-44.44% and average content was 3.43-3.89 μg/kg.[Conclusion]The SAs content order in 4 soils of vegetable plot was the vegetable land near the chicken farmthe vegetable land of ordinary farmhouseorganic green vegetable basegreen vegetable base,showing that the pollution of sulfonamides antibiotics was mainly derived from the manure application.

关 键 词：磺胺类抗生素 土壤 高效液相色谱 

分 类 号：X131.3[环境科学与工程—环境科学]