AQP1、AQP3和AQP8在子宫颈癌中的表达研究  

Research on expression of AQP1,AQP3 and AQP8 in cervical carcinoma

作　　者：史永华[1] 陈锐[2] 拉莱.苏祖克 

机构地区：[1]新疆医科大学基础医学院病理教研室,新疆乌鲁木齐830011 [2]新疆医科大学第三附属医院妇科,新疆乌鲁木齐830014

出　　处：《实用肿瘤杂志》2012年第1期18-21,共4页Journal of Practical Oncology

基　　金：国家自然科学基金地区基金(81060216)

摘　　要：目的探讨AQP1、AQP3和AQP8在子宫颈癌中的表达及意义。方法通过RT-PCR和免疫荧光检测AQP1、AQP3和AQP8在人子宫颈癌细胞系SiHa中的表达,通过免疫组织化学方法检测AQP1、AQP3和AQP8在35例维吾尔族子宫颈癌、15例CIN3和15例排除子宫颈癌及癌前病变对照组中的表达。结果 (1)SiHa细胞中AQP1、AQP3和AQP8在mRNA和蛋白水平均表达;(2)AQP1在对照组、CIN3和子宫颈癌组的微血管密度分别是43.6±17.8、56.2±11.6、70.8±21.1,3组间差异有统计学意义(P<0.05);AQP3在对照组、CIN3和子宫颈癌组的阳性率分别是13.3%、26.7%、48.6%,子宫颈癌组与对照组间差异有统计学意义(P<0.05);AQP8在3组间的阳性率分别是46.7%、86.7%、54.3%,3组间差异有统计学意义(P<0.05)。结论 AQP1、AQP3和AQP8的表达可能与子宫颈癌的发生、发展有关。Objective To probe expression and significance of AQP1,AQP3 and AQP8 in cervical carcinoma. Methods The expressions of AQP1,AQP3 and AQP8 in SiHa cell line were examined by RT-PCR and immunofluorescence and in 35 cases of cervical carcinoma,15 cases of CIN3 and 15 cases of control group by immunohistochemistry. Results（1） AQP1,AQP3 and AQP8 expressed in SiHa cell line;（2）MVD of AQP1 were 43.6±17.8,56.2±11.6,70.8±21.1 in control group,CIN3 and cervical carcinoma group,respectively.The difference among three groups was significant（P〈0.05）.Positive rates of AQP3 were 13.3%,26.7%,48.6%,respectively,in control group,CIN3 and cervical carcinoma group.The difference between cervical carcinoma and control group was significant（P〈0.05）.Positive rates of AQP8 were 46.7%,86.7%,54.3%,respectively,in three groups.The difference among three groups was significant（P〈0.05）. Conclusion Expression of AQP1,AQP3 and AQP8 in cervical carcinoma might be related with cervical carcinogenesis and development.

关 键 词：宫颈肿瘤 膜蛋白质类 肿瘤细胞 培养的 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]