富血小板血浆对人真皮细胞增殖及PDGF、TGF-β和VEGF表达的影响  

Effect of the platelet-rich plasma on the proliferation and the expression of PDGF,TGF-β and VEGF of human dermal fibroblasts in vitro

作　　者：王悦[1] 朱喆[2] 成荣杰[3] 张丽红[4] 马英智[5] 周延民[1] 

机构地区：[1]吉林大学口腔医院种植中心,长春130021 [2]吉林大学前卫医院病理科 [3]哈尔滨医科大学第四临床医院妇产科 [4]吉林大学白求恩医学院病理系 [5]空军航空大学培训基地门诊部

出　　处：《实用口腔医学杂志》2012年第1期64-69,共6页Journal of Practical Stomatology

基　　金：吉林省科学技术厅项目(编号:200804423)

摘　　要：目的:研究富血小板血浆(PRP)对人真皮成纤维细胞(hFbs)增殖、分化及胞质中PDGF、TGF-β和VEGF表达量的影响。方法:2次离心法制备PRP,成骨诱导条件下hFbs培养液中加入不同浓度PRP于第12天、21天钙-钴法染色,第21天茜素红染色;免疫细胞化学法检测加不同浓度PRP后第4天细胞PDGF、TGF-β和VEGF的表达;碱性磷酸酶染色(ALP)检测细胞活性;CCK-8法检测有和无成骨诱导条件下不同浓度PRP对细胞增殖的影响。结果:茜素红染色显示,2.8%PRP组钙结节形成最明显;钙-钴法染色显示,2.8%PRP组矿化作用最强;免疫细胞化学结果表明PDGF表达存在剂量依赖性,TGF-β和VEGF表达虽无剂量依赖性,但有适宜浓度的要求;ALP染色表明3%PRP组细胞增殖明显,活性最强。CCK-8检测表明各浓度PRP对hFbs均有促增殖作用,PRP加成骨诱导组比单纯PRP组表现出更明显的促增殖作用,其中4.8%PRP促增殖作用最强。结论:适宜浓度的PRP可促进hFbs增殖,但相关因子的表达存在适宜浓度的要求,PRP通过促进细胞增殖,增加相关因子表达促进创伤愈合。Objective： To study the effects of platelet-rich plasma（PRP） on the proliferation and differentiation of human dermal fibroblasts（hFbs） and on the expression of platelet-derived growth factor（PDGF）,transforming growth factor（TGF-β） and vascular endothelial growth factor（VEGF） in hFbs.Methods： PRP was prepared by twice centrifugation method.hFbs were treated by different concentrations of PRP and cultured under osteogenesis induction medium.The mineralization of the hFbs was examined by calcium-cobalt staining and the alizarin red staining at 12 d and 21 d.The expression of PDGF,TGF-β and VEGF was evaluated by immunocytochemistry,alkaline phosphatase（ALP） expression was tested by ALP staining,cell proliferation was detected by CCK-8 assay.Results： The formation of calcium node in 2.8%PRP group in mineralization induction culture was significant.The expression of PDGF was PRP dose-dependence,but TGF-β and VEGF expression needed an appropriate PRP concentration.ALP expression increased in 3% PRP group significantly.Proliferation of hFbs in mineralization induction culture was increased more.Conclusion： PRP may promote the proliferation,differentiation and relative growth factor expression of hFbs.

关 键 词：富血小板血浆 人真皮成纤维细胞 血小板衍化生长因子 血管内皮细胞生长因子 转化生长因子 增殖 分化 

分 类 号：R363[医药卫生—病理学]