辛伐他汀对大鼠血管平滑肌细胞增殖及FGFR1与p21ras表达的干预作用  

Effect of simvastatin on the proliferation and the expression of FGFR1 and p21ras in rats vascular smooth muscle cells

作　　者：侯慧芳[1] 刘国庆[1] 王永玲[1] 孙银平[1] 郭勇[1] 

机构地区：[1]新乡医学院基础医学院病理生理学教研室,453003

出　　处：《实用医学杂志》2012年第3期367-369,共3页The Journal of Practical Medicine

基　　金：河南省教育厅自然科学基金资助项目(编号:2008A310012);河南省科技厅科技攻关课题基金资助项目(编号:0424410129)

摘　　要：目的:观察辛伐他汀对血管平滑肌细胞(VSMC)增殖中FGFR1、p21ras蛋白表达的影响。方法:体外培养大鼠VSMC,建立VSMC增殖模型,应用不同浓度的辛伐他汀干预,MTT法测定细胞增殖活力;免疫印迹法检测FGFR1与p21ras蛋白的表达。结果:与对照组比较,辛伐他汀各浓度组细胞增殖活力均显著降低(P<0.05);与对照组(FGFR1208.07±10.70,p21ras202.43±12.36)相比,低剂量组(FGFR1181.47±7.43,p21ras182.00±8.98)的FGFR1、p21ras表达差异无显著性(P>0.05),中剂量组(FGFR1144.90±9.03,p21ras147.03±9.13)、高剂量组(FGFR1104.43±8.08,p21ras100.3±8.37)的表达均较明显降低(P<0.05),中、高剂量组FGFR1、p21ras表达较低剂量组均显著降低(P<0.05),高剂量组表达又较中剂量组显著降低(P<0.05),呈剂量依赖性。结论:辛伐他汀能抑制VSMC增殖,该作用是通过抑制FGFR1、p21ras蛋白表达实现的,这可能是辛伐他汀治疗动脉粥样硬化、再狭窄及高血压等心血管疾病的重要机制之一。Objective To investigate the effect of simvastatin on the proliferations and the expressions of FGFR1 and p21ras in rats vascular smooth muscle cells （SMCs）. Methods SMCs were cultivated in vitro and VSMC models of proliferation were established in the study. The alive cells counting by tetrazolium dye-reduction assay（MTT） were use to determine the effect of simvastatin on VSMCs proliferation at different concentrations （0, 1, 5, 10 μmol/L）, the expressions of FGFR1 and p21ras were detected by Western blot methods. Results Compared with the control gruop, other simvastatin concentration groups were appeared different levels of growth inhibition （P 〈 0.05）; Compared with the control group, the expressions of FGFR1, p21ras was unconspicuously changed in low dose group while the expressions were more significantly reduced in middle dose and high dose groups （P 〈 0.05） ; Compared with middle dose group, the expression was clearly reduced in high dose group group （P 〈 0.05）, and there was a obvious dose dependency. Conclusions Simvastatin might play a role of inhibiting VSMCs proliferation by reducing the expression of FGFR1 and p21ras, it may be one of the important mechanisms that simvastatin treated cardiovascular disease such as atherosclerosis, restenosis and hypertension.

关 键 词：辛伐他汀 VSMC FGFR1 P21RAS 

分 类 号：R965[医药卫生—药理学]