HPLC测定11种中药口服液中6种防腐剂的含量  

Simultaneous Determination of Preservatives in Traditional Chinese Oral Liquid Preparation by HPLC

作　　者：孙蒙[1] 闫小玉[1] 毕开顺[1] 陈晓辉[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中国实验方剂学杂志》2012年第3期76-79,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立HPLC同时测定中药口服液体制剂中防腐剂含量的方法。方法:Kromasil C18色谱柱(4.6 mm×250mm,5μm),流动相乙腈-0.02 mol.L-1乙酸铵溶液梯度洗脱,流速1.0 mL.min-1,检测波长240 nm,柱温30℃。结果:苯甲酸、山梨酸、对羟基苯甲酸酯类在1.0～181.1 mg.L-1线性关系良好(r>0.999);平均回收率95.5%～100.8%(RSD 0.7%～1.5%)。结论:方法简单、可靠,精确,可用于中药口服液体制剂中防腐剂含量的测定。Objective： To establish a HPLC method for simultaneous determination of preservatives in traditional chinese oral liquid preparation.Method： The chromatographic separation was achieved on a Kromasil C18（4.6 mm×150 mm,5 μm） column with acetonitrile-0.02 mol·L-1 ammonium acetate solution as mobile phase（gradient elution）.The flow rate was 1.0 mL·min-1,the detective wavelength was set at 240 nm,and the column temperature was maintained at 30 ℃.Result： Benzoic acid,sorbic acid and parahydroxybenzoate ester have a good resolution and linear correlation in certain range,respectively（r0.999）.The average recoverieswere 95.5%-100.8%（RSD 1.5%-1.8%）.Conclusions： The method is simple,reliable and accurate for determination of preservatives in traditional chinese oral liquid preparation.

关 键 词：口服液体制剂 苯甲酸 山梨酸 对羟基苯甲酸酯 测定 

分 类 号：R284.1[医药卫生—中药学]