双水相体系与超声集成提取月季花总黄酮  

Separation of total flavonoids from Rosa chinensis Jacp by two-phase aqueous system synergism with ultrasound extraction

作　　者：张敏娜[1] 朱军[1] 王光辉[2] 王汀[1] 丁瑞芳[1] 张华丽[1] 

机构地区：[1]山东省济宁医学院药学院,日照276826 [2]山东省济宁医学院基础医学与法医学院,济宁272067

出　　处：《上海医药》2012年第3期29-30,52,共3页Shanghai Medical & Pharmaceutical Journal

基　　金：济宁医学院2008年青年科学基金资助项目

摘　　要：目的:研究月季花中总黄酮的最佳提取方法。方法:以月季花总黄酮提取率为指标,采用乙醇-硫酸铵双水相体系集成超声提取法提取月季花中总黄酮,通过单因素试验和正交试验优选其最佳提取方法。结果:最佳提取条件确定为料液比1:50,醇水比0.6,硫酸铵浓度0.27g/ml,超声提取50min。结论:方法操作简便、效率高,优于回流提取。Objective： To explore the optimal conditions for extraction of total flavones from Flos rosae Chinensis. Methods： The extraction rate of flavones from Flos rosae Chinensis was assigned to index,total flavones from Flos rosae Chinensis were extracted by alcohol-ammonium sulfate two-phase aqueous system synergism with ultrasound extraction. The extraction conditions were optimized by single experiment and orthogonal test. Results： The optimal condition of extraction process was as follows： material-solvent ratio 1 ：50,ethanol-water ratio 0.6,the concentration of ammonium sulfate 0.27 g/ml and extracting for 50 min with ultrasonic wave. Conclusion： The proposed method is simple and effective,and much better than reflux extraction with heating.

关 键 词：双水相 超声提取 月季花 总黄酮 

分 类 号：R284.2[医药卫生—中药学] TQ028[医药卫生—中医学]