小链霉菌HCCB10043产脂肽类抗生素的鉴定及其生物合成基因簇研究  

Identification of Lipopeptide Antibiotics and Their Biosynthetic Gene Cluster in Streptomyces parvus HCCB10043

作　　者：饶敏[1,2] 靳旭[1,2] 阮丽军[2] 罗敏玉[2] 盛下放[1] 

机构地区：[1]南京农业大学生命科学学院,江苏南京210095 [2]上海来益生物药物研究开发中心,上海200240

出　　处：《中国医药工业杂志》2012年第2期92-96,136,共6页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项(2009ZX9302-004)

摘　　要：使用高效液相色谱法分离纯化了小链霉菌HCCB10043的重要代谢产物,获得3个精制品;经高分辨质谱、二级质谱与氨基酸分析鉴定其分别为A21978C1、C2和C3。菌株16S rDNA与已知的A21978C产生菌玫瑰孢链霉菌NRRL11379的同源性为99.2%,系统发育分析显示两者的亲缘关系很近;经基因钓取与沉默验证,结果显示该菌株的A21978C生物合成基因簇与玫瑰孢链霉菌报道完全相同。An HPLC method, coupled with MS data analysis and amino acid analysis, was established for the separation and identification of the major metabolites from the fermentation broth of Streptomyces parvus HCCB10043. The obtained three compounds were identified as A21978C1, C2 and C3. The 16S rDNA sequence of S. parvus HCCB10043 showed 99.2% identity to that of S. roseosporus NRRLl1379, the known A21978C producer, and phylogenetic analysis revealed that they had very close relationship. Gene cluster for the biosynthesis of A21978C complex in S. parvus HCCB 10043 was identified by comparing with the reported one in S. roseosporus NRRL11379 as reference, and validated by gene in-frame deletion. The results showed that the ~ene clusters were same in both strains.

关 键 词：小链霉菌 A21978C 16SrDNA 生物合成基因簇 

分 类 号：R978.1[医药卫生—药品]