髌韧带内侧缺损构建兔膝骨关节炎模型初探  

Research on knee osteoarthritis model by medial laceration of patellar ligament in rabbits

作　　者：朱鸿飞[1] 冯伟[1] 刘益杰[1] 褚立希[1] 杨松滨[1] 张建坡[1] 

机构地区：[1]上海中医药大学康复医学院,上海201203

出　　处：《上海中医药杂志》2012年第1期11-14,18,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市自然科学基金资助项目(09ZR1432000);上海市浦江人才计划资助项目(109J1409300);上海高校创新团队(第二期)建设项目[沪教委科(2009)6;26];上海市浦东新区卫生系统重点学科群建设项目(PWZxkq2011-01)

摘　　要：目的探讨构建膝骨关节炎动物模型的方法。方法将16只健康纯种成年新西兰大白兔随机分为正常组、模型4周组、模型6周组和模型8周组,每组4只。各模型组实验兔于无菌条件下造成双后肢膝内侧髌韧带2/3缺损,术后允许其自由活动,手术7天后每天强迫活动1h。光镜下观察造模后各时间段股骨髁负重区软骨病理改变,ELISA试剂盒检测血清中MMP-13及TNF-α水平。结果模型6周组、模型8周组出现关节软骨细胞变化、基质分解破坏、滑膜增生等炎症及退行性改变,其Mankin评分及血清MMP-13、TNF-α水平与正常组差异均有统计学意义(P<0.05);模型6周组表现为膝骨关节炎早期改变,模型8周组表现为膝骨关节炎中期改变。结论髌韧带内侧缺损法构建兔膝骨关节炎模型,方法可行,能更好地再现因应力异常而导致的膝骨关节炎。Objective To build knee osteoarthritis model by medial laceration of patellar ligament in rabbits. Methods Sixteen rabbits were randomized into normal group and model groups （4th week, 6th week and 8th week）, four rabbits in each group. Twelve rabbits in model groups underwent the operation to lead to the two-thirds laceration of patellar ligament in rabbits＇ hind legs, and the rabbits were forced to move freely since 7th day after operation, one hour per day. Pathological changes of cartilage in femoral condyle weight area were observed at different times, and the serum MMP-13 level and TNF-α level were detected by ELISA. Results Changes of osteoarthritis in model group at 6th week and 8th week after operation showed articular cartilage altered, matrix decomposition failure and synovial hyperplasia; the difference in Mankin score, serum MMP-13 levels and TNF-α levels between normal group and model groups was significant （P 〈 0.05 ）. The osteoarthritis was changed at early stage in the sixth week model group, and changed at medium stage in the eighth week model group. Conclusion Osteoarthritis modeling in rabbits by this way is feasible and resembles the osteoarthritis by abnormal stress.

关 键 词：膝骨关节炎 疾病模型 兔 髌韧带内侧缺损 

分 类 号：R-332[医药卫生] R684