椒目仁油中α-亚麻酸的鉴定和含量测定研究  

Study on the Identification and Content Determination of α-linolenic Acid in the Send oil from Zanthoxylum bungeanum Maxim

作　　者：曹蔚[1] 王四旺[1] 杨倩[1] 郝蓉蓉[1] 李瑛[1] 寸丽[1] 

机构地区：[1]第四军医大学药学系天然药物学教研室,西安710032

出　　处：《科学技术与工程》2012年第3期494-496,共3页Science Technology and Engineering

基　　金：陕西省"13115"科技创新工程技术研究中心基金(S2010ZDGC105)资助

摘　　要：对椒目仁油的鉴定及有效成分测定方法进行研究,为药材及制剂标准的制定提供试验依据。采用薄层色谱法对椒目仁油中α-亚麻酸进行定性鉴别;用高效液相色谱法对其有效成分α-亚麻酸进行含量测定。结果是α-亚麻酸在(20～400)μg.mL-1范围内呈良好线性关系。α-亚麻酸平均回收率为98.80%;5批椒目仁油中α-亚麻酸的平均含量为21.70%。为椒目仁油制剂标准的制订提供了参考。所建立的方法操作简便、准确可靠、重现性好,能有效控制椒目仁油的质量。To identify and analysis the content of α-linolenic acid in the send oil from Zanthoxylum bungeanum Maxim,the scientific foundation for the quality control and standard establishment of Zanthoxylum bungeanum Maxim are provided.TLC was used for identification of α-linolenic acid.The contents of α-linolenic acid were determined by HPLC.It is resulted that linear range of α-linolenic acid was（20~400）μg·mL-1（r=0.999 8）.The average recovery of α-linolenic acid was 98.80%.The content of α-linolenic acid in the send oil from Zanthoxylum bungeanum Maxim were 21.70%.These results are helpful to develop the quality standards of send oil from Zanthoxylum bungeanum Maxim.The method is accurate,simple and reproducible which can be applied to the quality control.

关 键 词：椒目仁油 Α-亚麻酸 薄层色谱 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学] R927.1[医药卫生—中医学]