地塞米松联合谷氨酰胺灌肠对溃疡性结肠炎大鼠血清IL-6和TNF-α的影响  

Changes of IL-6 and TNF-α in serum of ulcerative colitis rat after clysis by dexamethasone combine glutamine

作　　者：傅苏娜[1] 

机构地区：[1]绍兴市第六人民医院消化内科,浙江绍兴312000

出　　处：《全科医学临床与教育》2012年第1期12-13,21,共3页Clinical Education of General Practice

摘　　要：目的观察采用地塞米松联合谷氨酰胺灌肠治疗溃疡性结肠炎大鼠后血清炎症因子IL-6和TNF-α的变化情况,初步探讨两种药物联合灌肠的作用机制。方法采用三硝基苯磺酸(TNBS)/乙醇制作溃疡性结肠炎Sprague-Dawley(SD)大鼠模型。分别设立正常对照组、模型对照组、联合治疗组(地塞米松+谷氨酰胺灌肠)和地塞米松组(地塞米松单独灌肠),给药7d后取血,以酶联免疫吸附法(ELISA)测定各组大鼠血清IL-6和TNF-α的变化情况。结果模型组、联合治疗组和地塞米松组血清IL-6和TNF-α水平均高于正常对照组(P均<0.05),联合治疗组血清中IL-6和TNF-α均低于模型组和地塞米松组(P均<0.05)。结论地塞米松联合谷氨酰胺灌肠优于地塞米松单独灌肠,可有效降低溃疡性结肠炎大鼠血清IL-6和TNF-α水平,抑制促炎因子的释放。Objective To find out the effect of clysis by dexamethasone combine glutamine on the level of IL-6 and TNF-α in serum of ulcerative colitis rat. Methods UC rat model was induced by administration of trinitro-benzenesulfonic acid（TNBS）and alcohol. Normal control group, model control group, combined drug group and dexamethasone group were set up, and each group had at least 7 rats. The ELISA kits were used to detect the IL-6 and TNF-α in serum. Results The contents of IL-6 and TNF-α in model control group, combined drug group and dexamethasone group were all more than the normal control group with significant difference （P〈0.05）. The concentration in combined drug group were lower than that in the model control and dexamethasone groups （P〈O.05）. Conclusions The treatments of clysis by dexamethasone combine glutamine are more effective than dexamethasone clyster alone in controling the level of IL-6 and TNF-α in serum.

关 键 词：地塞米松 谷氨酰胺 灌肠 溃疡性结肠炎 

分 类 号：R574.62[医药卫生—消化系统]