显微激光散射技术对不同氧合状态下地贫红细胞的研究  

Microscope laser light scattering studies on red cells of thalassemia with image processing and analysis

作　　者：李平阳[1] 黄耀熊[2] 王江成[1] 谭润初[1] 李永龙[1] 贺奇才[1] 

机构地区：[1]中山医科大学物理教研室,广东广州510089 [2]暨南大学生物医学工程研究所,广东广州510632

出　　处：《中国医学物理学杂志》2000年第1期21-23,共3页Chinese Journal of Medical Physics

基　　金：国家教委博士点基金项目下的部分工作;中山医校科研基金资助项目!(223069623)

摘　　要：报道应用显微准弹性激光散射（ＭＱＬＳ）技术与显微生物医学图像分析技术对不同氧合情况下的单个活态正常人和较重症地中海贫血患者红细胞的研究。在实验中，对正常人及较重症（地中海贫血患者红细胞胞内血红蛋白聚集体的平均流体力学半径、红细胞膜的搏动频率等动态特性参数，以及对应的细胞的截面积、规化形状因子、长径、短径等图像分析数据同时进行了测定。发现正常人红细胞在氧合与去氧情况下，各种参数都没有显著性差异，但在低氧分压下，地贫红细胞膜的Γｓ在细胞表面积／体积比率降低的情况下大大下降，膜变形能力也相应下降，使之容易被阻滞、吞噬、消灭。Microscopic quasi-elastic laser light scattering technique with image processing and analysis were used to study normal and major thalassemia human erythrocytes in different oxygen pressure. The mean hydrodynamic radius of hemoglobin polymers inside the intact red cells, the membrane motion frequency, cross area, regular form factor, longest axis and shortest axis were measured for both kinds of erythrocytes. It is found that the membrane motion of normal human erythrocyte is similar in both oxygenated and deoxygenated states. While the membrane motion of (-thalassemia erythrocytes slows down in deoxygenated state.

关 键 词：图象分析 地中海贫血 MQLS 地贫红细胞 

分 类 号：R556.61[医药卫生—血液循环系统疾病]