RNAi沉默PSA基因对人前列腺癌细胞系LNCaP生长的影响  

Inhibition of growth of human prostate cancer cell line LNCaP by silencing the PSA gene using RNA interference

作　　者：徐建明[1] 何永忠[1] 雷鸣[1] 吴文起[1] 李逊[1] 黄锦昆[1] 杨后猛[1] 桂志明[1] 钟文[1] 李天[1] 曾国华[1] 李舒珏[1] 

机构地区：[1]广州医学院港湾医院广东省泌尿外科重点实验室,广东广州510230

出　　处：《海南医学》2012年第1期1-3,共3页Hainan Medical Journal

基　　金：广东省科学技术研究基金(编号:A2008307)

摘　　要：目的研究运用RNA干扰技术沉默PSA基因后对人前列腺癌细胞系LNCaP细胞产生的影响。方法构建针对PSA基因的小分子干扰RNA(PSA-siRNA),将前列腺癌LNCaP系进行分组,设PSA-siRNA转染组、阴性对照组、转染液组(只由转染试剂进行转染实验)和对照组(不添加任何转染试剂)进行实验。应用蛋白印迹法检测PSA的变化,并通过MTT法和流式细胞技术检测LNCaP细胞的调亡和细胞增殖方面的变化。结果蛋白印迹法检测结果显示,与阴性对照组、转染液和对照组比较,应用PSA-siRNA处理48h后的LNCaP细胞株,PSA的表达明显降低,组间比较差异有统计学意义(P<0.05)。MTT法和流式细胞仪检测示,转染组的细胞生长抑制率为27.5%,且观察到更强的细胞调亡(19.3%)。结论运用RNA干扰技术沉默PSA基因可以有效抑制LNCaP细胞中PSA基因的表达,进而抑制LNCaP细胞的生长与增殖,并可有效诱导细胞调亡。Objective To investigate the change of the growth of human prostate cancer cell line LNCaP by silencing PSA genes using RNA interference. Methods Small interfering RNA for PSA gene （PSA-siRNA） was initially constructed. The LNCaP ceils were divided into four groups, including PSA-siRNA transfection group, negative control group, transfeetion reagents group and the control group. Then the expression of PSA was determined by western blot. The apoptosis and proliferation of LNCaP ceils were detected by MTT assay and flow cytom- etry. Results After treatment with PSA-siRNA for 48 h, the expression of PSA was significantly decreased compared with the negative control group, transfection reagents group and the control group （P〈0.05）. PSA-siRNA transfection group has the inhibition rate of 27.5% and 19.3% of cell apoptosis. Conclusion Silencing the PSA gene by RNAi can actively suppress the expression ofPSA gene, and inhibit the growth and proliferation of LNCaP cells.

关 键 词：PSA RNA干扰 LNCAP细胞 细胞调亡 细胞增殖 

分 类 号：R737.25[医药卫生—肿瘤]