卡介苗提取物体外诱导小鼠脾淋巴细胞对人膀胱癌细胞的杀伤作用  

Killing effect of mouse spleen lymphocytes induced by active BCG extracts to human bladder cancer cell in vitro

作　　者：蒋翡翎[1] 单保恩[2] 姚敏[1] 魏小斌[1] 伍燕[1] 邓碧兰[1] 

机构地区：[1]海口市人民医院检验科,海南海口570208 [2]河北医科大学第四医院科研中心,河北石家庄050011

出　　处：《海南医学》2012年第1期18-20,共3页Hainan Medical Journal

摘　　要：目的分离活卡介苗(Bacille Calmette-Guérin,BCG)的有效组分,分析其有效组分诱导的小鼠脾淋巴细胞体外对人膀胱癌细胞株T24细胞的杀伤作用。方法采用酶裂解法和超声波破碎法对活BCG进行裂解,通过Sephadex G100凝胶层析对其有效组分进行分离及分子量的测定,用SDS-PAGE凝胶电泳进行纯度鉴定;采用四氮唑盐(MTT)法测定BCG有效组分BCGE2诱导的小鼠脾淋巴细胞对人膀胱癌细胞株T24细胞的杀伤活性。结果 BCGE2可体外诱导小鼠脾淋巴细胞对膀胱癌细胞株T24细胞的杀伤作用,在效靶比为20:1时对T24细胞的抑制率为42.31%,而对照组仅为13.32%,两者比较差异有统计学意义(P<0.01)。结论 BCGE2诱导的小鼠脾淋巴细胞对人膀胱癌细胞株T24的杀伤作用很显著,且随着效靶比的增加而增强。Objective To exlract, isolate, purify and identify the active components of Bacille Calmette-Guerin （BCG）, and to analyze the killing effect of mouse spleen lymphocytes induced by active BCG extracts to human bladder cancer cell line T24 in vitro. Methods Live BCG was divided by the enzyme and ultrasonic method to extract the active protein. The components of BCG extracts was isolated, and analyzed for the molecular weight by Sephadex gel filtration chromatography, then further studied to identify the purity and the molecular weight by the SDS-PAGE gelatin electrophoresis. The killing activity of mouse spleen lymphocytes induced by the active component BCGE2 to the human bladder cancer cell line T24 was analyzed by MTT method. Results The mouse spleen lymphocytes induced by BCGE2 could kill the bladder cancer cell line T24 in vitro. When the effect cells to the target ceils was 20：1, the inhibit rate was 42.31%, which was significantly higher than 13.32% in the control group （P〈0.01）. Conclusion The mouse spleen lymphocytes induced by BCGE2 have obvious killing effect on the bladder cancer cell T24 in dose-dependent manner.

关 键 词：BCG提取物 脾淋巴细胞 膀胱癌细胞株T24细胞 杀伤作用 

分 类 号：R-332[医药卫生]