用PCR-SSP作HLA-B位点分型及B22亚型分析  

HLA-B locus genotyping and B22 subtyping by PCR-SSP method

作　　者：杨颖[1] 冯喆[1] 张雁征[1] 冯明亮[1] 嵇月华[1] 陆琼[1] 许一多[2] 

机构地区：[1]上海血液中心,上海200051 [2]白求恩医科大学第三临床医院,长春130021

出　　处：《中国免疫学杂志》2000年第2期91-94,共4页Chinese Journal of Immunology

基　　金：上海市卫生局青年科研基金!编号 13 195 4Y4

摘　　要：目的:用DNA分型弥补血清学HLAB位点分型的欠缺并了解南方汉族B22亚型分布情况。方法:采用标准细胞作参照,建立B位点的PCRSSP分型方法;并对血清学B22阳性标本进行B22亚型分析。结果:104株标准细胞PCRSSP分型结果与已知结果完全一致,17例白血病人及同胞分型与血清学一致,1例不符;B22亚型以B5401最多,B5601较少,1例分型格局异常,可能为新B22等位基因或错配的DNA双链。结论:PCRSSP可用于B位点的DNA分型,比血清学更直观精确,操作简便,不受疾病状态影响。Objectives:The purpose of this study is to provide a HLA B locus genotyping method which is expected to compensate the unsatisfactory serology B locus typing and to explore the distribution of B22 subtypes.Methods:Taking standard cell lines provided by the XIIth International HLA Workshop as reference,the authors established the PCR SSP method for HLA B genotyping.By this method,the HLA B alleles of leukemia patients were typed and 57 individuals previously identified as HLA B22 by serologic typing were genotyped.Results:The results of B locus genotyping of 104 cell lines by PCR SSP and that of reported were completely concordant .Unambiguous results of 17 leukemia patients and their relations were gotten except one discordant from serology which was thought as mistaken serotyping .Out of 57 samples ,55 were confirmed to bear B54 or B55 or B56 allele,respectively ,with B54 being the most common allelic form,and another showed a unique amplifying pattern which was different from any known HLA B22 alleles so far reported, suggesting a new allele or 'mismatched ”DNA double strands which needed further study .Conclusions:PCR SSP was proven to be a practical genotyping method for B locus because of its simplicity, rapidity ,accuracy and unvariability with changes of health.

关 键 词：HLA-B PCR-SSP 分型 B22亚型 白血病 

分 类 号：R733.7[医药卫生—肿瘤]