诱发性肝癌雄性激素受体的动态变化及氟他胺对受体表达和成癌的影响  

Change of androgen receptor in induced hepatoma and influence of flutamide on expression of androgen receptor and hepatocarcinogenesis

作　　者：周载平[1] 梁力建[2] 

机构地区：[1]中山医科大学第一附属医院肝胆外科,广州510080 [2]中山市人民医院普外科,528400

出　　处：《中华普通外科杂志》2000年第1期39-42,共4页Chinese Journal of General Surgery

摘　　要：目的　探讨肝癌癌变过程中雄性激素受体的动态变化及氟他胺 (flutamide)对受体和成癌的影响。方法　将 95只SD大鼠分 3组构建诱癌模型。A组 :单纯二甲氨基偶氮苯诱癌。B组 :诱癌加氟他胺治疗。C组 :空白对照。第 1～ 5个月各组取 3～ 5只鼠称体重、肝重 ,取肝组织、癌灶及癌周组织作病理学检查并以放射配基结合法检测雄性激素受体。结果　 (1)B组肝体比值上升比A组延迟 1个月 (P <0 0 5 ) ,癌分化程度较高。 (2 )A组雄性激素受体浓度以癌变启动期 (第 1个月 )和成癌期 (第 5个月 )最高 ,第 2个月最低 ,细胞核定量分别为 (5 6± 9)、(5 9± 4)、(34± 3)fmol/mg·蛋白质。B组受体分别为 (37± 7)、(38± 7)、(5 6± 3)fmol/mg·蛋白质 (P <0 0 5 ) ,C组的生理性表达与年龄依赖的性活动能力一致。 (3)A组雄激素受体以肝细胞型肝癌最高〔胞核定量 (5 9± 5 )fmol/mg·蛋白质〕 ,且肝癌组织大于肝癌周肝组织 (P <0 0 5 )。结论　癌变过程雄性激素受体有明显的时间和空间特点 ,雄性激素敏感期的拮抗治疗可能抑制雄性激素的促增殖效应。Objects[WT5”BZ] To investigate changes of androgen receptor(AR) in hepatocarcinogenesis and influence of flutamide on the expression of AR and carcinogenesis.[WT5”HZ] Methods[WT5”BZ] 95 male SD rats were divided into group A receiving (3' Me DAB), B(3' Me DAB+Flutamide),and C(control group).After the inducement of liver cancer three to five rats of each group were sacrificed to measure body weight,liver weight,undergo pathological examination of liver tissue or tumor tissue and detect AR every month.[WT5”HZ] Results[WT5”BZ] (1)The rise of liver to body weigh ratio occurred 1 month later in group B than in group A( P <0 05), and tumors in group B had a better differentiation compared with those of group A.(2) Nucleus AR in group A was at the highest level at early stage of induction (the 1st month)and tumor forming stage(the 5th month),being (56±9) and (59±4) fmol/mg·protein, respectively, and at the lowest level at the 2th month being (34±3) fmol/mg·protein. In contrast, it was the lowest at the 1st and the highest level at the 2th month, being (37±7) and (38±7) fmol/mg·protein, respectively, and at highest level at the 2th month, being (56±3) fmol/mg·protein in group B. In group C, the physiological expression of AR was in accordance with age dependent sex activity.(3) Of different histological type of induced tumor in group A, hepatocellular carcinoma was at the highest level of AR ( P <0 05), and the level of AR was significantly higher in tumor tissue than in tumor surrounding tissue( P <0 05).[WT5”HZ] Conclusions[WT5”BZ] Change of AR in hepatocarcinogenesis have an obvious spatio temporal character. Early administration of androgenantagonist in sensitive phase has inhibitory role on the proliferation promoting effect of androgen. [WT5”HZ]

关 键 词：肝细胞癌 受体 雄激素 雄激素拮抗剂 

分 类 号：R735.7[医药卫生—肿瘤]