CHD5与KLF5蛋白在胃癌组织中的表达及其与预后的关系  

Association of CHD5 and KLF5 expression with prognosis in gastric carcinoma

作　　者：罗登[1] 赵公芳[1] 路明亮[1] 黄华[1] 常江[1] 郑梦瑶[1] 

机构地区：[1]昆明医学院第二附属医院消化内科,云南省昆明市650101

出　　处：《世界华人消化杂志》2011年第35期3603-3609,共7页World Chinese Journal of Digestology

基　　金：云南省卫生科技计划基金资助项目;No.2010NS066~~

摘　　要：目的:探讨胃癌组织中CHD5和KLF5的表达意义及其与预后的关系.方法:收集2000-01/2007-06在昆明医学院第二附属医院手术切除的208例胃癌组织标本,采用免疫组织化学SP法检测胃腺癌组织、癌旁组织中CHD5和KLF5的表达;采用χ2检验和Fisher精确概率法分析胃癌中CHD5和KLF5的表达与临床病理指标的相关性;采用Cox比例风险模型方法进行多因素分析;应用Kaplan-Meier生存分析法判断CHD5和KLF5表达与各临床病理参数及其预后的关系.结果:胃癌组织中CHD5和KLF5呈明显低表达特点.其中,CHD5的表达与患者年龄、肿瘤分化程度、浸润深度、TNM分期、淋巴结和远处转移等有关,差异有统计学意义(P<0.05);KLF5的表达与肿瘤分化程度、浸润深度、TNM分期、淋巴结和远处转移等有关,差异有统计学意义(P<0.05).多因素分析显示,患者的生存时间主要受患者性别、肿瘤部位、组织分化程度、远处转移、TNM分期、CHD5和KLF5的异常表达等因素影响;Kaplan-Meier生存分析显示,CHD5、KLF5阴性组的中位生存期分别为(21.00mo±1.36mo)、(20.00mo±1.54mo),而CHD5、KLF5阳性组的中位生存期分别为(55.00mo±6.97mo)、(45.00mo±3.27mo).CHD5和KLF5阴性表达组患者的1、3年生存率明显低于阳性表达患者,差异均有统计学意义.结论:胃癌组织中CHD5和KLF5低表达与胃癌的侵袭转移及不良预后存在相关性.提示CHD5和KLF5蛋白的异常表达可能参与胃黏膜恶性转变以及胃癌发生发展等生物学过程.AIM： To investigate the expression of chromodomain helicase DNA-binding protein 5 （CHD5） and Krüppel-like factor 5 （KLF5） in gastric cancer, and to evaluate whether CHD5 and KLF5 can be used as prognostic markers in gastric cancer. METHODS： Immunohistochemistry staining was performed to detect the expression of CHD5 and KLF5 proteins in 208 surgical specimens of gastric cancer and 68 noncancerous gastric tissue specimens. The association of CHD5 and KLF5 expression in gastric cancer with the survival time of patients was retrospectively analyzed. RESULTS： Reduced expression of CHD5 and KLF5 frequently occurred in gastric cancer. The positive rates of CHD5 and KLF5 expression in gastric cancer were 29.33% （61/208） and 38.46% （80/208）, respectively. CHD5 expression was correlated with age, histologic differentiation, depth of invasion, regional lymph node metastasis, distant metastasis, and TNM stage （all P 0.05）. KLF5 expression was correlated with histologic differentiation, depth of invasion, lymph node metastasis, distant metastasis, and TNM stage （all P 0.05）. Further multivariate analysis revealed that patient＇s gender, tumor location, histologic differentiation, distant metastasis, TNM stage, and expression of CHD5 and KLF5 were independent prognostic factors in patients with gastric cancer. The Kaplan-Meier plot showed that the median survival was 21.00 ± 1.36 months in patients with negative expression of CHD5 and 20.00 ± 1.54 months in those with negative expression of KLF5. The median survival time was 55.00 ± 6.97 months in patients with positive CHD5 expression and 45.00 ± 3.27 months in patients with positive KLF5 expression. The cumulative 1- and 3-year survival rates were significantly lower in patients with negative expression of CHD5 and KLF5 than in those with positive expression of these two proteins. CONCLUSION： Reduced expression of CHD5 and KLF5 in gastric cancer is associated with tumor metastasis and poor survival. Ectopic expression of CHD5 and KLF5 p

关 键 词：胃癌 CHD5蛋白 KLF5蛋白 临床病理特征 预后 

分 类 号：R735.2[医药卫生—肿瘤]