南蛇藤总萜抑制荷肝癌HepA1-6小鼠移植瘤的生长  

Celastrus orbiculatus extract inhibits the xenograft tumor growth of HepA1-6 hepatoma in mice

作　　者：侯莹[1,2] 员林[2,3] 钱亚云 张华[4] 刘延庆 

机构地区：[1]溧水县人民医院肿瘤科,南京211200 [2]扬州大学中西医结合研究所,扬州225009 [3]中国人民解放军第97医院影像科,徐州221000 [4]扬州大学医学院护理系,扬州225009

出　　处：《肿瘤》2011年第11期999-1003,共5页Tumor

摘　　要：目的:探讨南蛇藤总萜对荷肝癌HepA1-6小鼠移植瘤生长的影响及其可能机制。方法:建立肝癌HepA1-6小鼠移植瘤模型,分为空白对照组、溶媒对照组(1%DMSO)、阴性对照组(0.9%氯化钠溶液)、阳性对照组(顺铂)及南蛇藤总萜低(10 mg/kg)、中(20 mg/kg)和高(40 mg/kg)剂量组。观察南蛇藤总萜对小鼠体质量、抑瘤率、肝脏指数、脾脏指数及胸腺指数的影响;应用免疫组织化学法检测移植瘤组织中血管内皮生长因子(vascular end othelial growth factor,VEGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的表达以及微血管密度(microvessel density,MVD)计数;TUNEL法检测移植瘤组织中的细胞凋亡。结果:南蛇藤总萜可明显抑制荷肝癌HepA1-6小鼠移植瘤的生长,促进肿瘤细胞凋亡,下调移植瘤组织中VEGF和bFGF的表达以及MVD计数,与阴性对照组比较,差异有统计学意义(P<0.05);南蛇藤总萜组小鼠肝、脾和胸腺指数及体质量与阴性对照组比较,差异无统计学意义(P>0.05)。结论:南蛇藤总萜可明显抑制荷肝癌HepA1-6小鼠移植瘤的生长,可能与其促进肿瘤细胞凋亡及抑制肿瘤血管生成有关。Objective：To investigate the effect of Celastrus orbiculatus extracts（COE） on xenograft tumor growth of HepA1-6 hepatoma in mice,and to explore its possible mechanism.Methods：HepA1-6 hepatoma model was established in mice,and then these mice were randomly divided into blank control group,solvent control group（1% DMSO）,negative control group（0.9% NaCl solution）,positive control group（cisplatin）,and low-,medium-and high-dose COE groups（10,20 and 40 mg/kg,respectively）.The body weight,tumor growth inhibitory rate,liver index,spleen index and thymus gland index were calculated,and the expression levels of vascular endothelial growth factor（VEGF） and basic fibroblast growth factor（bFGF） as well as microvessel density（MVD） were detected by immunohistochemistry.The apoptosis rate was determined by TUNEL assay.Results：COE could significantly inhibit the tumor growth,increase the apoptosis rate,and down-regulate the expression levels of VEGF,bFGF and MVD of HepA1-6 hepatoma in the liver of mice,as compared with the negative control group（P0.05）.There was no statistically significant differences in liver index,spleen index,thymus gland index and body weight between COE-treated groups and the negative control group（P0.05）.Conclusion：COE can inhibit the xenograft tumor growth of HepA1-6 hepatoma in mice.This effect may be associated with the enhancement of apoptosis and the inhibition of angiogenesis in tumors.

关 键 词：肝肿瘤 模型 动物 新生血管化 病理性 南蛇藤 小鼠 

分 类 号：R735.7[医药卫生—肿瘤]